Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice

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README.md

Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice

This repository contains the ephys data related to the article " Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice". We provide optogenetic in vivo recordings (LFP and SUA data) from L2/3 of the prefrontal cortex of 72 not-anesthetized mice of three age groups of control and early stimulated (ES) mice expressing ChR2(ET/TC) in a subset of L2/3 pyramidal neurons achieved by in utero electroporation. Following the structure of the article, the dataset contains recordings of:

  • 11 P11-12 control mice
  • 10 P11-12 ES mice
  • 13 P23-25 control mice
  • 14 P23-25 ES mice
  • 12 P38-40 control mice
  • 12 P38-40 ES mice

Each group folder contains the corresponding recordings including LFP signals and sorted unit information including timestamps of optogenetic stimulation with 473 nm and 596 nm of 3 ms long 4 Hz pulse and 3s long ramp light stimulation.

LFP:

The group folders contain LFP.dat files with the signal traces of the corresponding recording with a sampling frequency of 1000 Hz recorded in L2/3 of the prelimbic subdivision of the prefrontal cortex. Each recording contains a baseline period followed by optogenetic stimulations according following protocol:

  1. 30 times, 3 s long, 596 nm stimulations
  2. 30 times, 3 s long, 473 nm stimulations
  3. 360 times, 3 ms long, 473 nm stimulations
  4. 360 times, 3 ms long, 596 nm stimulations

Please consider that stimulation timestamps and detection of unit action potentials have a higher sampling frequency of 32000 Hz.

SUA:

The group folders also contain SUA.mat files with information about manually sorted single units of the corresponding recording. In sum for all groups 5716 units were sorted. Spike sorting has been done using klusta (https://klusta.readthedocs.io/en/latest/), and manually curated with Phy (https://phy.readthedocs.io/en/latest/). For every recording, a MATLAB structure is present which provides information of following information for every sorted unit (Unit_Info):

  • ClusterID: ID of the unit
  • Channel: The channel on which the unit has been detected
  • Timestamps: Timestamps of detected action potential with a sampling frequency of 32000 Hz
  • Waveform: the average waveform of the unit

Electrode channels:

All recordings were recorded with a 16 channel Neuronexus 1x16 1-shank electrode with a channel interspace of 100 µm. Channel 1 is the most superficial (closer to brain surface), and 16 the deepest. The position of electrodes has been post-mortem histologycally reconstructed.

For every question and/or further data request, please don't hesitate in contacting me.

datacite.yml
Title Not-anesthetized ephys data associated with the paper *Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice*
Authors Bitzenhofer,Sebastian H.;: Center for Neural Circuits and Behavior, Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA;https://orcid.org/0000-0003-0736-6251
Pöpplau,Jastyn A.;Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;https://orcid.org/0000-0002-4350-3164
Chini,Mattia;Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;http://orcid.org/0000-0002-5782-9720
Marquardt,Annette;Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Hanganu-Opatz,Ileana L.;Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;https://orcid.org/0000-0002-4787-1765
Description Electrophysiological recordings from prefrontal cortex of 72 not-anesthetized mice: - 11 P11-12 control mice - 10 P11-12 ES mice - 13 P23-25 control mice - 14 P23-25 ES mice - 12 P38-40 control mice - 12 P38-40 ES mice
License Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References
Funding EU, ERC-2015-CoG 681577
DFG, Ha 4466/10-1
DFG, Ha 4466/11-1
DFG, Ha 4466/12-1
DFG, SFB 936 B5
DFG, SPP 1665
Keywords Neuroscience
Electrophysiology
Cognitive dysfunction
Early activity
Prefrontal cortex
Neurodevelopment
Mental disorders
LFP
SUA
Resource Type Dataset