Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice

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README.md

Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice

This repository contains the ephys data related to the article " Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice". We provide 239 optogenetic in vivo recordings of non-anesthetized and urethane-anesthetized mice of control and early stimulated (ES) mice expressing ChR2(ET/TC) in a subset of L2/3 pyramidal neurons in the right hemisphere of the prefrontal cortex achieved by in utero electroporation. Following the structure of the article, the dataset contains recordings of:

72 electrophysiological recordings from prefrontal cortex of non-anesthetized mice (Ephys_not-anesthetized):

  • 11x P11-12 control recordings
  • 10x P11-12 ES recordings
  • 13x P23-26 control recordings
  • 14x P23-26 ES recordings
  • 12x P38-40 control recordings
  • 12x P38-40 ES recordings

62 electrophysiological recordings from prefrontal cortex of urethane anesthetized mice (Ephys_anesthetized)

  • 10x P11-12 control recordings
  • 10x P11-12 ES recordings
  • 10x P23-25 control recordings
  • 11x P23-25 ES recordings
  • 9x P38-40 control recordings
  • 12x P38-40 ES recordings

21 electrophysiological recordings from prefrontal cortex with transcranial stimulations (Transcranial_recordings):

  • 13x P7-11 recordings
  • 8x P12-16 recordings

8 electrophysiological recordings from prefrontal cortex in PV cre-mice (PV_Photo-tagging):

  • 3x P26 recordings
  • 5x P37-40 recordings

25 electrophysiological recordings from prefrontal cortex and Hippocampus of non-anesthetized mice (ES_non-anesthetized_HPstim):

  • 13x P38-40 control recordings
  • 12x P38-40 ES recordings

23 electrophysiological recordings from prefrontal cortex of non-anesthetized mice (oldES_non-anesthetized):

  • 11x P38-40 control recordings
  • 12x P38-40 old ES recordings

28 electrophysiological recordings from prefrontal cortex during social preference task (ES_social_preference):

  • 15x P38-40 control recordings
  • 13x P38-40 ES recordings

For detailed information of group generation and recording protocol refer to the article.

LFP:The group folders contain LFP.dat files with the signal traces of the corresponding recording with a sampling frequency of 1000 Hz recorded in L2/3 of the right hemisphere of the prelimbic subdivision of the prefrontal cortex. Experiments in non-anesthetized mice with acute hippocampus stimulation include further LFP signal traces of the intermediate CA1 and folders of experiments in urethane anesthetized mice include further LFP signal traces of L2/3 and 5/6 of both hemispheres. All recorded mice, except PV cre-mice used for PV photo-tagging, express ChR2(ET/TC) in a subset of L2/3 pyramidal neurons in the right hemisphere of the prefrontal cortex.

Each recording contains a baseline period followed by optogenetic stimulations according following protocol:

  1. 3 s long, 596 nm stimulations (ramps)
  2. 3 s long, 473 nm stimulations (ramps)
  3. 3 ms long, 473 nm stimulations (square pulses)
  4. 3 ms long, 596 nm stimulations (square pulses)

For each recording a Matlab structure is provided containing the timestamps and kind of stimulation. Please consider that stimulation timestamps and detection of unit action potentials have a higher sampling frequency of 32000 Hz

SUA:The group folders also contain SUA.mat files with information about manually sorted single units of the corresponding recording. According to the article single unit information is provided for following datasets: Non-anesthetized ES and control mice of all three age groups, recordings with transcranial stimulations and optogenetic recordings in PV cre-mice. Spike sorting has been done using klusta (https://klusta.readthedocs.io/en/latest/), and manually curated with Phy (https://phy.readthedocs.io/en/latest/). For every recording, a Matlab structure is present which provides following information for every sorted unit (Unit_Info):

  • ClusterID: ID of the unit
  • Channel: The channel on which the unit has been detected
  • Timestamps: Timestamps of detected action potential with a sampling frequency of 32000 Hz
  • Waveform: the average waveform of the unit

All non-anesthetized recordings were recorded with a 16 channel Neuronexus 1x16 1-shank electrode with a channel interspace of 100 µm. Channel 1 is the most superficial (closer to brain surface), and 16 the deepest. The position of electrodes has been post-mortem histologycally reconstructed.

All anesthetized recordings were recorded with two contralateral inserted Neuronexus 4x4 4-shank electrodes. The position of electrodes has been post-mortem histologycally reconstructed.

For every question and/or further data request, please don't hesitate in contacting me.

datacite.yml
Title Not-anesthetized ephys data associated with the paper *Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice*
Authors Bitzenhofer,Sebastian H.;: Center for Neural Circuits and Behavior, Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA;https://orcid.org/0000-0003-0736-6251
Pöpplau,Jastyn A.;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;https://orcid.org/0000-0002-4350-3164
Chini,Mattia;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;http://orcid.org/0000-0002-5782-9720
Marquardt,Annette;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany
Hanganu-Opatz,Ileana L.;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;https://orcid.org/0000-0002-4787-1765
Description 72 electrophysiological recordings from prefrontal cortex of non-anesthetized mice: - 11x P11-12 control recordings - 10x P11-12 ES recordings - 13x P23-26 control recordings - 14x P23-26 ES recordings - 12x P38-40 control recordings - 12x P38-40 ES recordings 62 electrophysiological recordings from prefrontal cortex of anesthetized mice: - 10x P11-12 control recordings - 10x P11-12 ES recordings - 10x P23-25 control recordings - 11x P23-25 ES recordings - 9x P38-40 control recordings - 12x P38-40 ES recordings 21 electrophysiological recordings from prefrontal cortex with transcranial stimulations: - 13x P7-11 recordings - 8x P12-16 recordings 8 electrophysiological recordings from prefrontal cortex in PV cre-mice: - 3x P26 recordings - 5x P37-40 recordings 25 electrophysiological recordings from prefrontal cortex and Hippocampus of non-anesthetized mice: - 13x P38-40 control recordings - 12x P38-40 ES recordings 23 electrophysiological recordings from prefrontal cortex of non-anesthetized mice: - 11x P38-40 control recordings - 12x P38-40 old ES recordings 28 electrophysiological recordings from prefrontal cortex during social preference task: - 15x P38-40 control recordings - 13x P38-40 ES recordings
License Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References
Funding EU, ERC-2015-CoG 681577
DFG, Ha 4466/10-1
DFG, Ha 4466/11-1
DFG, Ha 4466/12-1
DFG, SFB 936 B5
DFG, SPP 1665
Keywords Neuroscience
Electrophysiology
Cognitive dysfunction
Early activity
Prefrontal cortex
Neurodevelopment
Mental disorders
Gamma
LFP
SUA
Resource Type Dataset