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Exp_001_SCstim_DiffLocations
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ReadMe.md

Exp_001_SCstim_DiffLocations

Experiment description:

Stimulating the Schaffer collateral pathway and 2Photon Linescan imaging of dendrites at different locations along the dendrites.

Aim: Is dendritic calcium after Schaffer collateral triggered plateau potential global or localised to specific dendritic sections.

Experiment conditions

  • Hippocampal brain slices (CA3 cut off)
  • in 50uM Picrotoxin

Stimulation:

  • Single stim followed by burst of 5 stim at 50hz
  • Stim intensity set to give a plateau potential
  • Stimulated 3-6 times with a linescan on each stimulation

ExIDs (date_slice-id_cell-id):

  • 201204_s2_c1
  • 201207_s1_c1
  • 201209_s1_c1
  • 201214_s1_c1
  • 201216_s1_c1
  • 210325_s1_c1
  • 210325_s1_c2

Experiments by: Matt Udakis