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confocal Z-stacks and analysis files of murine brain slices from different brain regions, fluorescently labeled by transgenic markers (glutamaergic, gabaergic and serotonergic neurons) or immunomarkers (NeuN, TH for dopaminergic Neurons, Iba-1 for microglia).

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Christiane Nolte 95b677639e Dateien hochladen nach '' 2 years ago
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MOD_Raphe und SN.pzfx 4483929c98 updated files final version 2 years ago
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README.md

Satellite_microglia

Imaging files and analysis files relevant for publication Bakina et al "Microglia form satellites with different neuronal subtypes in the adult murine CNS" Murine brain slices from different brain regions were fluorescently labeled by transgenic markers (glutamaergic, gabaergic and serotonergic neurons) or immunomarkers (NeuN, TH for dopaminergic Neurons, Iba-1 for microglia). Confocal Z-stacks were acquired on a Zeiss LSM 700 and 3 D volumes analysed for microglia-neuron soma contacts by IMARIS (surface-to-surface tool) or ImageJ in 3 D volumes.

datacite.yml
Title Satellite Microglia in the mouse CNS # Additional information about the resource, e.g., a brie| Microglia are the immune cells of the central nervous system. A subpopulation, satellite microglia, form direct contact of their cell body to a neuron cell body. This particular neuron-glia interaction may have implications for neuron function. To understand what factors determine satellite microglia formation we performed a morphological analysis of adult mouse brains and retinae. Satellite microglia occur in all areas containing neuron somata, and their frequency is determined by the soma density of neurons in a given brain region whereas a preference to form a satellite with neurons of a specific neurotransmitter subtype was not observed.
Authors Nolte,Christiane;Cellular Neurosciences, Max Delbrueck Center for Molecular medicine, Berlin, Germany;ORCID:0000-0001-5398-3440
Kettenmann,Helmut;Shenzhen Institute of Advanced Technology, Chin.Acad.Sci, Shenzhen, China;ResearcherID:X-1234-5678
Bakina,Olga
License Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References Manuscript in submission [] (IsSupplementTo)
[] ()
Funding Institutional funding
Keywords Neuroscience
microglia
neuron-glia interaction
satellite microglia
GABAergic
glutamatergic
serotonergic
dopaminergic
image analysis
Resource Type Dataset