README.md 2.6 KB
Anesthesia_CA1
This repository contains data related to the article "Anesthetics fragment hippocampal network activity, alter spine dynamics and affect memory consolidation" (https://www.biorxiv.org/content/10.1101/2020.06.05.135905v1). Published in PLOS Biology doi: 10.1371/journal.pbio.3001146.
We provide calcium imaging and electrophysiology data from hippocampal CA1 during wakefulness, sleep and under various general anesthetics (Isoflurane, Keta/Xyl and MMF).
| Awake | Isoflurane | Keta/Xyl | MMF |
|---|---|---|---|
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Following the structure of the repository, the dataset contains:
- LFP and SUA recordings from 4 mice under Isoflurane, Keta/Xyl and MMF anesthesia (electrophysiology folder)
- 189 distinct 2-photon calcium imaging recordings from 7 different mice under Isoflurane, Keta/Xyl and MMF anesthesia (calcium imaging folder)
- LFP and SUA recordings in awake and sleep conditions (with sleep scoring) from 4 and 3 mice, respectively (Sleep Data/LFP and Sleep Data/SUA folders), 1-2 hours of duration.
- 74 distinct 2-photon calcium imaging recordings from 3 mice in awake and sleep conditions (Sleep Data/calcium imaging folder)
This repository contains electrophysiological data and processed calcium imaging recordings. Raw calcium imaging recordings are available in another repository.
Data structure info
Most data structure should be self explanatory / inferrable from the description in the manuscript, but some useful information is provided in the respective "meta_data" excel files.
Miscellaneous
- information about the numpy calcium imaging files can be found in the suite2p documentation page (https://suite2p.readthedocs.io/en/latest/)
- for LFP data, only the CA1 reversal channel is provided
- LFP data has a sampling rate of 320 Hz and has been band-pass filtered in the 0.1-160 Hz range
- code used for the analysis and statistics together with stats-dataset can be found at https://github.com/mchini/Yang_Chini_et_al and https://github.com/OpatzLab/HanganuOpatzToolbox
Typos and bugs reported by users
- In Fig. 1 and Table 2 of the data descriptor, the duration of the electrophysiological recordings is indicated as fixed to 1.5 hours, while in reality, it varied from recording to recording in different conditions.