# Anesthesia_CA1

This repository contains data related to the article "Anesthetics fragment hippocampal network activity, alter spine dynamics and affect memory consolidation" (https://www.biorxiv.org/content/10.1101/2020.06.05.135905v1). Published in PLOS Biology doi: [10.1371/journal.pbio.3001146](https://doi.org/10.1371/journal.pbio.3001146).

We provide calcium imaging and electrophysiology data from hippocampal CA1 during wakefulness, sleep and under various general anesthetics (Isoflurane, Keta/Xyl and MMF).


**Awake**             |  **Isoflurane**       |  **Keta/Xyl**   |   **MMF**   
:-------------------------:|:-------------------------:|:-------------------------:|:-------------------------:
![](repo_figures/51_awake_256_256.gif)  |  ![](repo_figures/51_iso_256_256.gif) | ![](repo_figures/51_keta_256_256.gif) | ![](repo_figures/51_MMF_256_256.gif) 

Following the structure of the repository, the dataset contains:
- LFP and SUA recordings from 4 mice under Isoflurane, Keta/Xyl and MMF anesthesia (electrophysiology folder)
- 189 distinct 2-photon calcium imaging recordings from 7 different mice under Isoflurane, Keta/Xyl and MMF anesthesia (calcium imaging folder)
- LFP and SUA recordings in awake and sleep conditions (with sleep scoring) from 4 and 3 mice, respectively (Sleep Data/LFP and Sleep Data/SUA folders), 1-2 hours of duration.
- 74 distinct 2-photon calcium imaging recordings from 3 mice in awake and sleep conditions (Sleep Data/calcium imaging folder)

This repository contains electrophysiological data and processed calcium imaging recordings. Raw calcium imaging recordings are available in [another repository](https://gin.g-node.org/SW_lab/Calcium_imaging_CA1_raw).

## Data structure info

Most data structure should be self explanatory / inferrable from the description in the manuscript, but some useful information is provided in the respective "meta_data" excel files.

### Miscellaneous

- information about the numpy calcium imaging files can be found in the suite2p documentation page (https://suite2p.readthedocs.io/en/latest/)
- for LFP data, only the CA1 reversal channel is provided
- LFP data has a sampling rate of 320 Hz and has been band-pass filtered in the 0.1-160 Hz range
- code used for the analysis and statistics together with stats-dataset can be found at https://github.com/mchini/Yang_Chini_et_al and https://github.com/OpatzLab/HanganuOpatzToolbox

### Typos and bugs reported by users

- In Fig. 1 and Table 2 of the data descriptor, the duration of the electrophysiological recordings is indicated as fixed to 1.5 hours, while in reality, it varied from recording to recording in different conditions.