Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice
This repository contains the ephys data related to the article " Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice". We provide 134 optogenetic in vivo recordings of not-anesthetized and urethane-anesthetized mice of three age groups of control and early stimulated (ES) mice expressing ChR2(ET/TC) in a subset of L2/3 pyramidal neurons in the right hemisphere of the prefrontal cortex achieved by in utero electroporation. Following the structure of the article, the dataset contains recordings of:
Not-anesthetized
- 11 recordings of P11-12 control mice
- 10 recordings of P11-12 ES mice
- 13 recordings of P23-25 control mice
- 14 recordings of P23-25 ES mice
- 12 recordings of P38-40 control mice
- 12 recordings of P38-40 ES mice
For all not-anesthetized recordings LFP signals, sorted unit information and timestamps of optogenetic stimulation with 473 nm and 596 nm of 3 ms long 4 Hz pulse and 3s long ramp light stimulation are provided.
LFP: The group folders contain LFP.dat files with the signal traces of the corresponding recording with a sampling frequency of 1000 Hz recorded in L2/3 of the right hemisphere of the prelimbic subdivision of the prefrontal cortex. Each recording contains a baseline period followed by optogenetic stimulations according following protocol:
- 30 times, 3 s long, 596 nm stimulations (ramps)
- 30 times, 3 s long, 473 nm stimulations (ramps)
- 360 times, 3 ms long, 473 nm stimulations (square pulses)
- 360 times, 3 ms long, 596 nm stimulations (square pulses)
Please consider that stimulation timestamps and detection of unit action potentials have a higher sampling frequency of 32000 Hz
SUA: The group folders also contain SUA.mat files with information about manually sorted single units of the corresponding recording. In sum for all not-anesthetized groups 5716 units were sorted. Spike sorting has been done using klusta (https://klusta.readthedocs.io/en/latest/), and manually curated with Phy (https://phy.readthedocs.io/en/latest/).
For every recording, a MATLAB structure is present which provides following information for every sorted unit (Unit_Info):
- ClusterID: ID of the unit
- Channel: The channel on which the unit has been detected
- Timestamps: Timestamps of detected action potential with a sampling frequency of 32000 Hz
- Waveform: the average waveform of the unit
All not-anesthetized recordings were recorded with a 16 channel Neuronexus 1x16 1-shank electrode with a channel interspace of 100 µm. Channel 1 is the most superficial (closer to brain surface), and 16 the deepest. The position of electrodes has been post-mortem histologycally reconstructed.
Urethane-anesthetized:
- 10 recordings of P11-12 control mice
- 10 recordings of P11-12 ES mice
- 10 recordings of P23-25 control mice
- 11 recordings of P23-25 ES mice
- 9 recordings of P38-40 control mice
- 12 recordings of P38-40 ES mice
All anesthetized recordings were recorded with two contralateral inserted Neuronexus 4x4 4-shank electrodes. The position of electrodes has been post-mortem histologycally reconstructed. The group folders contain LFP.dat files with the signal traces of the corresponding recording with a sampling frequency of 1000 Hz recorded in L2/3 and L5/6 of the right and left hemisphere of the prelimbic subdivision of the prefrontal cortex. Each recording contains a baseline period followed by optogenetic stimulations according following protocol:
- 30 times, 3 s long, 596 nm stimulations (ramps)
- 30 times, 3 s long, 473 nm stimulations (ramps)
- 180 rounds of 3 ms long square pulses in different frequencies for 3s with 473 nm
- 180 rounds of 3 ms long square pulses in different frequencies for 3s with 596 nm
Stimulation timestamps are provided in the corresponding MATLAB structure for each recording.
For every question and/or further data request, please don't hesitate in contacting me.
