Transférer les fichiers vers '2021-01-12'

2021-01-12/2021-01-12.txt

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+Date: 2021-01-12
+Experiment: WT DREADD awake DG + CA3
+Experimenter: Catherine
+Objective: in awake
+	4x16 probe in DG
+	32 linear 50 um probe in CA3 at 20 deg angle
+SURGERIES
+
+Mouse 1: 4399 - 0 dot , DOB 13-12-2020 , 23.9 g
+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
+Solutions: ACSF from 2018
+12:40	iso induction + bupre 
+12:50	Injection site = (0,0,0)==> DG marks (0.5;-0.5), (-0.5;0.5), 
+	CA3 marks at (1.5;0.25),(0.25,1.5) ==> center (0.65;0.82). 
+13:10	craniotomies very good
+13:20	Recovery 
+
+
+RECORDINGS
+
+Mouse 1: 4399 - 0 dot , DOB 13-12-2020 , 23.9 g
+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
+
+14:10	Removed the silicon, OK
+14:40	zero Q631 in the middle of the DG craniotomy 
+	zero CCO5 in the middle of the CA3 craniotomy
+	take impedances
+	Look for DS on probe Q631, ideqlly up in the depest shamks, and down on the shallow.
+	Q631 (-0.047;0.139,-2.31)
+15;10	CA1
+	Heard layer around 1550-1640
+	SWR go up above the layer, and down below CA1. 
+	2310-- shank 4 up DS
+	2250-- shank 3 up DS
+	2150-- shank 2 up DS
+	2110-- shank 1 up DS
+	We want all up before 200uM
+15:50 	CCO5 (0;0;0,2365)
+	1530-1690 CA1
+	2200-2365 ca3
+16:10	Recording 1
+16:45	Inject 100uL saline + recording 2
+	A47 channel seems broken
+16:20 	Recording 3
+
+17:45	remove probes	
+17:50	iso 5% - sac
+
+
+
+
+Notes for histology:
+4399
+CA3: 20 deg, DiI (1*1 marks total)
+DG: 0 deg, DiI x (1*4 marks total)
+TdTomato
+
+
+
+
+

2021-01-12/CM_210112_161439/2020011201.sea

@@ -0,0 +1 @@
+4

2021-01-12/CM_210112_161439/2020011201_01.cpt

@@ -0,0 +1,14 @@
+C:\POLY_WHEEL\Exercises\GluK2\run 30 min.xls
+#E
+Animal:
+Treatment:
+Date:
+Gene:
+Dose:
+Way of inj:
+Solvent:
+Product2:
+Lesion:
+Note:
+#FE
+WH_T=2294

2021-01-12/CM_210112_161439/2020011202_01.cpt

@@ -0,0 +1,14 @@
+C:\POLY_WHEEL\Exercises\GluK2\run 30 min.xls
+#E
+Animal:
+Treatment:
+Date:
+Gene:
+Dose:
+Way of inj:
+Solvent:
+Product2:
+Lesion:
+Note:
+#FE
+WH_T=0

2021-01-12/CM_210112_161439/amplifier.nrs

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+    <channel>95</channel>
+   </channelsShown>
+  </display>
+ </displays>
+</neuroscope>

2021-01-12/CM_210112_161439/digitalin.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s72638720--ad8617d76ca861b853f4483f5c17222d

2021-01-12/CM_210112_164631/2020011201_02.cpt

@@ -0,0 +1,14 @@
+C:\POLY_WHEEL\Exercises\GluK2\run 30 min.xls
+#E
+Animal:
+Treatment:
+Date:
+Gene:
+Dose:
+Way of inj:
+Solvent:
+Product2:
+Lesion:
+Note:
+#FE
+WH_T=1985

2021-01-12/CM_210112_164631/digitalin.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s72349440--da4d1735294395471cf45c7c9447091c

2021-01-12/CM_210112_171646/2020011201_03.cpt

@@ -0,0 +1,14 @@
+C:\POLY_WHEEL\Exercises\GluK2\run 30 min.xls
+#E
+Animal:
+Treatment:
+Date:
+Gene:
+Dose:
+Way of inj:
+Solvent:
+Product2:
+Lesion:
+Note:
+#FE
+WH_T=1056

2021-01-12/CM_210112_171646/digitalin.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s72390400--a275b1ef8130c47de4e138861b9737b0