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- Date: 2021-01-12
- Experiment: WT DREADD awake DG + CA3
- Experimenter: Catherine
- Objective: in awake
- 4x16 probe in DG
- 32 linear 50 um probe in CA3 at 20 deg angle
- SURGERIES
- Mouse 1: 4399 - 0 dot , DOB 13-12-2020 , 23.9 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- Solutions: ACSF from 2018
- 12:40 iso induction + bupre
- 12:50 Injection site = (0,0,0)==> DG marks (0.5;-0.5), (-0.5;0.5),
- CA3 marks at (1.5;0.25),(0.25,1.5) ==> center (0.65;0.82).
- 13:10 craniotomies very good
- 13:20 Recovery
- RECORDINGS
- Mouse 1: 4399 - 0 dot , DOB 13-12-2020 , 23.9 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- 14:10 Removed the silicon, OK
- 14:40 zero Q631 in the middle of the DG craniotomy
- zero CCO5 in the middle of the CA3 craniotomy
- take impedances
- Look for DS on probe Q631, ideqlly up in the depest shamks, and down on the shallow.
- Q631 (-0.047;0.139,-2.31)
- 15;10 CA1
- Heard layer around 1550-1640
- SWR go up above the layer, and down below CA1.
- 2310-- shank 4 up DS
- 2250-- shank 3 up DS
- 2150-- shank 2 up DS
- 2110-- shank 1 up DS
- We want all up before 200uM
- 15:50 CCO5 (0;0;0,2365)
- 1530-1690 CA1
- 2200-2365 ca3
- 16:10 Recording 1
- 16:45 Inject 100uL saline + recording 2
- A47 channel seems broken
- 16:20 Recording 3
- 17:45 remove probes
- 17:50 iso 5% - sac
- Notes for histology:
- 4399
- CA3: 20 deg, DiI (1*1 marks total)
- DG: 0 deg, DiI x (1*4 marks total)
- TdTomato
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