2021_Dreadd_awake_DGsp_CA3lfp/2021-01-12/2021-01-12.txt

Date: 2021-01-12
Experiment: WT DREADD awake DG + CA3
Experimenter: Catherine
Objective: in awake
	4x16 probe in DG
	32 linear 50 um probe in CA3 at 20 deg angle
SURGERIES

Mouse 1: 4399 - 0 dot , DOB 13-12-2020 , 23.9 g
injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
Solutions: ACSF from 2018
12:40	iso induction + bupre 
12:50	Injection site = (0,0,0)==> DG marks (0.5;-0.5), (-0.5;0.5), 
	CA3 marks at (1.5;0.25),(0.25,1.5) ==> center (0.65;0.82). 
13:10	craniotomies very good
13:20	Recovery 


RECORDINGS

Mouse 1: 4399 - 0 dot , DOB 13-12-2020 , 23.9 g
injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 

14:10	Removed the silicon, OK
14:40	zero Q631 in the middle of the DG craniotomy 
	zero CCO5 in the middle of the CA3 craniotomy
	take impedances
	Look for DS on probe Q631, ideqlly up in the depest shamks, and down on the shallow.
	Q631 (-0.047;0.139,-2.31)
15;10	CA1
	Heard layer around 1550-1640
	SWR go up above the layer, and down below CA1. 
	2310-- shank 4 up DS
	2250-- shank 3 up DS
	2150-- shank 2 up DS
	2110-- shank 1 up DS
	We want all up before 200uM
15:50 	CCO5 (0;0;0,2365)
	1530-1690 CA1
	2200-2365 ca3
16:10	Recording 1
16:45	Inject 100uL saline + recording 2
	A47 channel seems broken
16:20 	Recording 3

17:45	remove probes	
17:50	iso 5% - sac




Notes for histology:
4399
CA3: 20 deg, DiI (1*1 marks total)
DG: 0 deg, DiI x (1*4 marks total)
TdTomato