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@@ -0,0 +1,61 @@
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+Date: 2021-01-12
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+Experiment: WT DREADD awake DG + CA3
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+Experimenter: Catherine
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+Objective: in awake
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+ 4x16 probe in DG
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+ 32 linear 50 um probe in CA3 at 20 deg angle
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+SURGERIES
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+
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+Mouse 1: 4399 - 0 dot , DOB 13-12-2020 , 23.9 g
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+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
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+Solutions: ACSF from 2018
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+12:40 iso induction + bupre
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+12:50 Injection site = (0,0,0)==> DG marks (0.5;-0.5), (-0.5;0.5),
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+ CA3 marks at (1.5;0.25),(0.25,1.5) ==> center (0.65;0.82).
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+13:10 craniotomies very good
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+13:20 Recovery
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+
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+
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+RECORDINGS
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+
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+Mouse 1: 4399 - 0 dot , DOB 13-12-2020 , 23.9 g
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+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
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+
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+14:10 Removed the silicon, OK
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+14:40 zero Q631 in the middle of the DG craniotomy
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+ zero CCO5 in the middle of the CA3 craniotomy
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+ take impedances
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+ Look for DS on probe Q631, ideqlly up in the depest shamks, and down on the shallow.
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+ Q631 (-0.047;0.139,-2.31)
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+15;10 CA1
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+ Heard layer around 1550-1640
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+ SWR go up above the layer, and down below CA1.
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+ 2310-- shank 4 up DS
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+ 2250-- shank 3 up DS
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+ 2150-- shank 2 up DS
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+ 2110-- shank 1 up DS
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+ We want all up before 200uM
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+15:50 CCO5 (0;0;0,2365)
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+ 1530-1690 CA1
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+ 2200-2365 ca3
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+16:10 Recording 1
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+16:45 Inject 100uL saline + recording 2
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+ A47 channel seems broken
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+16:20 Recording 3
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+
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+17:45 remove probes
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+17:50 iso 5% - sac
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+
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+
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+
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+
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+Notes for histology:
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+4399
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+CA3: 20 deg, DiI (1*1 marks total)
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+DG: 0 deg, DiI x (1*4 marks total)
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+TdTomato
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+
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+
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+
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+
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+
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