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@@ -0,0 +1,73 @@
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+Date: 2021-01-22
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+Experiment: WT DREADD awake DG + CA3
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+Experimenter: Mariela
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+Objective: in awake
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+ 4x16 probe in DG
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+ 32 linear 50 um probe in CA3 at 20 deg angle
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+
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+SURGERIES
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+
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+Mouse : 4405 - 1 dot , DOB 20-11-2020 , 27.9 g
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+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
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+Solutions: serum phy from Upcare
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+
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+
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+10:40 iso induction + bupre
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+11:20 Injection site = (0,0,0) ==> DG marks (0.5;-0.5), (-0.5;0),
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+ CA3 marks at (1.5;0.25), (0.25,1.5) ==> center (0.74;0.77).
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+12:20 craniotomies good - small bledding in ca3
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+12:25 Recovery
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+
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+
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+RECORDINGS
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+
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+
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+Mouse : 4405 - 2 dot , DOB 20-11-2020 , 27.9 g
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+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
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+
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+14:05 Removed the silicon, OK, little oedema on DG craniotomy
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+15:00 zero S404 in the middle of the DG craniotomy
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+ zero CCO5 in the middle of the CA3 craniotomy
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+ take impedances
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+ CCO5 was bended yesterday, it looks like the 4 last electrodes (B28/B008,B29/B052,B30/,B31/)are broken
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+ (the last two were already broken from previous)
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+ Look for DS on probe S404, ideally up in the depest shanks, and down on the shallow.
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+ S404 (0.21;-0.14;-1.3)
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+15:30 The head bar wasn't well fixed. we took out the probes, fixed the bar and re introduced the probes.
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+ Litlle more oedema on DG craniotomy because we had to poke twice
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+ S404 (0.02;-0.2;-2.9)
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+15:20 CA1
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+ 1760-2120 CA1
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+ SWR go up above the layer, and down below CA1.
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+ DS go down above the layer and up in the 2/3rd of the layer itself
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+ All deepst channel to be up and all the shallow to be down
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+ We want all up before uM
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+ granule cell layer - spikes on :
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+ 2740-2940- shank 1
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+ 2810-2940- shank 2
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+ 2820-2940- shank 3
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+ 2830-2940- shank 4
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+ Unsure if it's real spikes or artefact when the mouse is moving.
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+ Reversal seems to happen within the upper part of the DG cellular layer.
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+15:55 CCO5 (0.99;0.02;)
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+ 1500-missed then end CA1
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+ stopped spiking, got silent so I don't think we are in CA2. We are rather confident we hit CA3.
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+ CA3
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+ 2220-2420
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+ Stopped spiking at 2290 but still ripples
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+16:55 Recording 1
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+17:25 Inject 200uL saline + recording 2
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+17:55 Recording 3
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+18:30 remove probes
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+18:40 K/X 0.3ulIP - sac
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+
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+Notes for histology:
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+4406
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+CA3: 20 deg, DiI (2*1 marks total, one only 800um deep)
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+DG: 0 deg, DiI x (2*4 marks total, one to (0.21;-0.14;-1.3), the other more medial at (0.02;-0.2;) )
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+TdTomato
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+
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+
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