2021_Dreadd_awake_DGsp_CA3lfp/2021-01-22/2021-01-22.txt

Date: 2021-01-22
Experiment: WT DREADD awake DG + CA3
Experimenter: Mariela
Objective: in awake
	4x16 probe in DG
	32 linear 50 um probe in CA3 at 20 deg angle

SURGERIES

Mouse : 4405 - 1 dot , DOB 20-11-2020 , 27.9 g
injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
Solutions: serum phy from Upcare


10:40	iso induction + bupre 
11:20	Injection site = (0,0,0) ==> DG marks (0.5;-0.5), (-0.5;0), 
	CA3 marks at (1.5;0.25), (0.25,1.5) ==> center (0.74;0.77). 
12:20	craniotomies good - small bledding in ca3
12:25	Recovery 


RECORDINGS


Mouse : 4405 - 2 dot , DOB 20-11-2020 , 27.9 g
injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 

14:05	Removed the silicon, OK, little oedema on DG craniotomy 
15:00	zero S404 in the middle of the DG craniotomy 
	zero CCO5 in the middle of the CA3 craniotomy
	take impedances
	CCO5 was bended yesterday, it looks like the 4 last electrodes (B28/B008,B29/B052,B30/,B31/)are broken 
	(the last two were already broken from previous)
	Look for DS on probe S404, ideally up in the depest shanks, and down on the shallow.
	S404 (0.21;-0.14;-1.3)
15:30	The head bar wasn't well fixed. we took out the probes, fixed the bar and re introduced the probes.
	Litlle more oedema on DG craniotomy because we had to poke twice
	S404 (0.02;-0.2;-2.9)
15:20	CA1
	1760-2120 CA1
	SWR go up above the layer, and down below CA1. 
	DS go down above the layer and up in the 2/3rd  of the layer itself
	All deepst channel to be up and all the shallow to be down
	We want all up before uM
	granule cell layer - spikes on :
	2740-2940- shank 1
	2810-2940- shank 2
	2820-2940- shank 3
	2830-2940- shank 4
	Unsure if it's real spikes or artefact when the mouse is moving.
	Reversal seems to happen within the upper part of the DG cellular layer.
15:55 	CCO5 (0.99;0.02;)
	1500-missed then end CA1
	stopped spiking, got silent so I don't think we are in CA2. We are rather confident we hit CA3.
	CA3
	2220-2420
	Stopped spiking at 2290 but still ripples
16:55	Recording 1
17:25	Inject 200uL saline + recording 2
17:55 	Recording 3
18:30	remove probes	
18:40	K/X 0.3ulIP - sac

Notes for histology:
4406
CA3: 20 deg, DiI (2*1 marks total, one only 800um deep)
DG: 0 deg, DiI x (2*4 marks total, one to (0.21;-0.14;-1.3), the other more medial at (0.02;-0.2;) )
TdTomato