Transférer les fichiers vers '2021-01-21'

2021-01-21/2021-01-21.txt

@@ -0,0 +1,70 @@
+Date: 2021-01-21
+Experiment: WT DREADD awake DG + CA3
+Experimenter: Mariela
+Objective: in awake
+	4x16 probe in DG
+	32 linear 50 um probe in CA3 at 20 deg angle
+
+SURGERIES
+
+Mouse : 4406 - 2 dot , DOB 20-11-2020 , 25 g
+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
+Solutions: serum phy from Upcare
+
+
+10:50	iso induction + bupre 
+11:20	Injection site = (0,0,0)very posterior in the ring ==> DG marks (0.5;-0.5), (-0.5;0), 
+	the ring was too anterior, impossible to do the DG mark at the right spot 
+	CA3 marks at (1.5;0.25), (0.25,1.5) ==> center (0.70;0.76). 
+12:30	craniotomies good - little oedema in DG cranio
+12:35	Recovery 
+
+
+RECORDINGS
+
+
+Mouse : 4407 - 3 dot , DOB 20-11-2020 , 25.7 g
+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
+
+13:50	Removed the silicon, OK
+14:30	zero S404 in the middle of the DG craniotomy 
+	zero CCO5 in the middle of the CA3 craniotomy
+	take impedances
+	Look for DS on probe S404, ideally up in the depest shanks, and down on the shallow.
+	S404 (0;0;-2.62)
+15:20	CA1
+	1290-1430 CA1
+	SWR go up above the layer, and down below CA1. 
+	DS go down above the layer and up in the 2/3rd  of the layer itself
+	All deepst channel to be up and all the shallow to be down
+	We want all up before uM
+	granule cell layer - spikes on :
+	2060-2300- shank 1
+	2110-2300- shank 2
+	2210-2300- shank 3
+	2240-2300- shank 4
+	Spikes on all the shanks :)
+	Reversal seems to happen within the upper part of the DG cellular layer.
+15:55 	CCO5 (0;0;-2.5)
+	missed begining-1600 CA1 
+	CA3
+	2100-2300
+	Stopped spiking at 2290 but still ripples
+16:00	Recording 1
+16:30	Inject 100uL CNO + recording 2
+	A47 channel seems broken
+	CC05 appears bended, no extra noise/broken channel at this point.
+17:00 	Recording 3
+17:50	remove probes	
+17:55	iso 5% - sac
+
+Notes for histology:
+4406
+CA3: 20 deg, DiI (1*1 marks total)
+DG: 0 deg, DiI x (1*4 marks total)
+TdTomato
+
+
+
+
+

2021-01-21/ME_210121_155701/101_01.cpt

@@ -0,0 +1,14 @@
+C:\POLY_WHEEL\Exercises\GluK2\run 30 min.xls
+#E
+Animal:
+Treatment:
+Date:
+Gene:
+Dose:
+Way of inj:
+Solvent:
+Product2:
+Lesion:
+Note:
+#FE
+WH_T=483

2021-01-21/ME_210121_155701/amplifier.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s6949109760--ba88445ea428e16fdeae7d8890d6fc31

2021-01-21/ME_210121_155701/digitalin.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s72386560--bc4d37d0b9c2331b5a4d705e87da20b5

2021-01-21/ME_210121_155701/time.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s144773120--2b9bd1da31c06a46267b5b336567d3a4

2021-01-21/ME_210121_162955/101_02.cpt

@@ -0,0 +1,14 @@
+C:\POLY_WHEEL\Exercises\GluK2\run 30 min.xls
+#E
+Animal:
+Treatment:
+Date:
+Gene:
+Dose:
+Way of inj:
+Solvent:
+Product2:
+Lesion:
+Note:
+#FE
+WH_T=480

2021-01-21/ME_210121_162955/amplifier.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s6944563200--fc8f712dcbd9107dbf3c375112024a34

2021-01-21/ME_210121_162955/digitalin.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s72339200--65d689a65819bb1db527d60e07b97efb

2021-01-21/ME_210121_162955/time.dat

@@ -0,0 +1 @@
+/annex/objects/MD5-s144678400--d7471cdcb0c5f67e8cd0029ae5581f04

2021-01-21/ME_210121_170006/101_03.cpt

@@ -0,0 +1,14 @@
+C:\POLY_WHEEL\Exercises\GluK2\run 30 min.xls
+#E
+Animal:
+Treatment:
+Date:
+Gene:
+Dose:
+Way of inj:
+Solvent:
+Product2:
+Lesion:
+Note:
+#FE
+WH_T=408

2021-01-21/ME_210121_170006/101_03.dat

@@ -0,0 +1,928 @@
+21-Jan-21 5:00:08 PM
+C:\POLY_WHEEL\Exercises\GluK2\run 30 min.xls
+Animal:
+Treatment:
+Date:
+Gene:
+Dose:
+Way of inj:
+Solvent:
+Product2:
+Lesion:
+Note:
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2021-01-21/ME_210121_170006/amplifier.dat

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2021-01-21/ME_210121_170006/digitalin.dat

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2021-01-21/ME_210121_170006/time.dat

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