| Title |
In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector. |
| Authors |
Leboulle,Gérard;Freie Universität Berlin, Neurobiologie;ORCID:0000-0001-9942-9795
Gehne,Nora;Freie Universität Berlin, Neurobiologie
Froese,Anja;Freie Universität Berlin, Neurobiologie
Menzel,Randolf;Freie Universität Berlin, Neurobiologie
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| Description |
In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells.
|
| License |
Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
|
| References |
Citation1 [doi:tba] (IsSupplementTo)
|
| Funding |
DFG, LE1809/1-1
DFG, LE1809/1-2
|
| Keywords |
Neuroscience
Insect
Apis mellifera
Honeybee
in-vivo electroporation
viral transduction
eGFP
|
| Resource Type |
Dataset
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Gérard Leboulle