T2A-GAL4_Library

Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors

This repository contains confocal data of T2A-GAL4 knock-in lines used for investigating the cellular organization and function of neurotransmitter receptor systems in the fly brain. Flies and experimental conditions should be referred to:

Kondo S., Takahashi T., Yamagata N., Imanishi Y., Katow H., Hiramatsu S., Lynn K., Abe A., Kumaraswamy A., Tanimoto H. (2019) "Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors". In: Cell Reports.

General Notes

1. Confocal dataset of Drosophila brains for GAL4 expression inserted in endogenous receptor genes. Expression is visualized with reporters for plasma membrane and nuclei with counterstaining of neuropils.
2. Each gene folder contains multiple subfolders with different labellings. These are named "UAS-myr-GFP_UAS-His-RFP", "UAS-myr-GFP_UAS-His-RFP", and "UAS-mCD8-GFP_brp-SNAP_IS2_Registration".

Folder Specific Notes

UAS-myr-GFP_UAS-His-RFP

It contains a few (typically three) full-brain multi-channel TIFF stacks visualized by myristoylated GFP (Pfeiffer et al. Genetics 2010) and His2A::RFP (Pandey et al. J Cell Sci, 2005). Ch1: myr::GFP, Ch2: His2A::RFP.

UAS-mCD8-GFP_brp-SNAP

It contains a few (typically three) central brain multi-TIFF stacks visualized by mCD8::GFP (Pfeiffer et al. Genetics 2010) together with neuropil counterstaining by SNAP-tagged active zone protein Bruchpilot (Kohl et al. PNAS 2014). Ch1: brp::SNAP, Ch2: mCD8::GFP.

UAS-mCD8-GFP_brp-SNAP_IS2_Registration

It contains central brain NRRD stacks derived from registering confocal data in the "UAS-mCD8-GFP_brp-SNAP" folder into the IS2 standard brain (Cachero et al., Curr Biol., 2010) by Computational Morphometry Toolkit (http://hdl.handle.net/10380/3140). "no1", "no2", "no3" in the file name stand for different individuals. "_01": brp-SNAP channel, "_02": GFP channel.