We use cookies to ensure you get the best experience on our website
Почетна Преглед Помоћ News
Регистрација Пријавите се
Tanimoto_lab
/
T2A-GAL4_Library
DOI
10.12751/g-node.10246f
6
0
Креирај огранак 2
Датотеке Дискусије 0 Захтеви за спајање 0 Вики

Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors

Грана: master
Гране Ознаке
T2A-GAL4_Lib...
Ajayrama Kumaraswamy d27e23de78 Update 'datacite.yml' пре 4 година
5-HT1B e9102e0f5d all files uploaded пре 4 година
5-HT2A e9102e0f5d all files uploaded пре 4 година
5-HT2B e9102e0f5d all files uploaded пре 4 година
5-HT7 e9102e0f5d all files uploaded пре 4 година
AkhR e9102e0f5d all files uploaded пре 4 година
AstA-R1 e9102e0f5d all files uploaded пре 4 година
AstA-R2 e9102e0f5d all files uploaded пре 4 година
AstC-R1 e9102e0f5d all files uploaded пре 4 година
AstC-R2 e9102e0f5d all files uploaded пре 4 година
CCAP-R e9102e0f5d all files uploaded пре 4 година
CCHa1-R e9102e0f5d all files uploaded пре 4 година
CCHa2-R e9102e0f5d all files uploaded пре 4 година
CCKLR-17D1 e9102e0f5d all files uploaded пре 4 година
CCKLR-17D3 e9102e0f5d all files uploaded пре 4 година
CG12344 e9102e0f5d all files uploaded пре 4 година
CG13229 e9102e0f5d all files uploaded пре 4 година
CG13575 e9102e0f5d all files uploaded пре 4 година
CG13995 e9102e0f5d all files uploaded пре 4 година
CG32547 e9102e0f5d all files uploaded пре 4 година
CG33639 e9102e0f5d all files uploaded пре 4 година
CG43795 e9102e0f5d all files uploaded пре 4 година
CG7589 e9102e0f5d all files uploaded пре 4 година
CapaR e9102e0f5d all files uploaded пре 4 година
CrzR e9102e0f5d all files uploaded пре 4 година
Dh-31R e9102e0f5d all files uploaded пре 4 година
Dh44-R2 e9102e0f5d all files uploaded пре 4 година
Dop1R1 e9102e0f5d all files uploaded пре 4 година
Dop1R2 e9102e0f5d all files uploaded пре 4 година
Dop2R e9102e0f5d all files uploaded пре 4 година
DopEcR e9102e0f5d all files uploaded пре 4 година
GABA-B-R1 e9102e0f5d all files uploaded пре 4 година
GABA-B-R2 e9102e0f5d all files uploaded пре 4 година
GluCLα e9102e0f5d all files uploaded пре 4 година
GluRIB e9102e0f5d all files uploaded пре 4 година
GluRIIA e9102e0f5d all files uploaded пре 4 година
GluRIIB e9102e0f5d all files uploaded пре 4 година
GluRIIC e9102e0f5d all files uploaded пре 4 година
GluRIID e9102e0f5d all files uploaded пре 4 година
GluRIIE e9102e0f5d all files uploaded пре 4 година
Grd e9102e0f5d all files uploaded пре 4 година
Gyc76C e704f595bc reorganized existing tiff files into folder UAS-myr-GFP_UAS-His-RFP пре 4 година
KaiR1D e9102e0f5d all files uploaded пре 4 година
Lcch3 e9102e0f5d all files uploaded пре 4 година
Lgr1 e9102e0f5d all files uploaded пре 4 година
Lgr3 e9102e0f5d all files uploaded пре 4 година
Lgr4 e9102e0f5d all files uploaded пре 4 година
NPFR e9102e0f5d all files uploaded пре 4 година
Nmdar1 e9102e0f5d all files uploaded пре 4 година
Nmdar2 e9102e0f5d all files uploaded пре 4 година
Oamb e9102e0f5d all files uploaded пре 4 година
Octβ2R e9102e0f5d all files uploaded пре 4 година
Octβ3R e9102e0f5d all files uploaded пре 4 година
PK1-R e9102e0f5d all files uploaded пре 4 година
PK2-R2 e9102e0f5d all files uploaded пре 4 година
Pdfr e9102e0f5d all files uploaded пре 4 година
RYa-R e9102e0f5d all files uploaded пре 4 година
SIFaR e9102e0f5d all files uploaded пре 4 година
SPR e9102e0f5d all files uploaded пре 4 година
TkR86C e9102e0f5d all files uploaded пре 4 година
TyrRII e9102e0f5d all files uploaded пре 4 година
hec e9102e0f5d all files uploaded пре 4 година
mAChR-A e9102e0f5d all files uploaded пре 4 година
mAChR-B e9102e0f5d all files uploaded пре 4 година
mGluR e9102e0f5d all files uploaded пре 4 година
mtt e9102e0f5d all files uploaded пре 4 година
nAChRα1 e9102e0f5d all files uploaded пре 4 година
nAChRα2 e9102e0f5d all files uploaded пре 4 година
nAChRα3 e9102e0f5d all files uploaded пре 4 година
nAChRα6 e9102e0f5d all files uploaded пре 4 година
nAChRα7 e9102e0f5d all files uploaded пре 4 година
nAChRβ1 e9102e0f5d all files uploaded пре 4 година
nAChRβ2 e9102e0f5d all files uploaded пре 4 година
nAChRβ3 e9102e0f5d all files uploaded пре 4 година
ort e9102e0f5d all files uploaded пре 4 година
rk e9102e0f5d all files uploaded пре 4 година
LICENSE 6a478a8636 Initial commit пре 5 година
README.md 285014d3af Update 'README.md' пре 4 година
datacite.yml d27e23de78 Update 'datacite.yml' пре 4 година

README.md

Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors

This repository contains confocal data of T2A-GAL4 knock-in lines used for investigating the cellular organization and function of neurotransmitter receptor systems in the fly brain. Flies and experimental conditions should be referred to:

Kondo S., Takahashi T., Yamagata N., Imanishi Y., Katow H., Hiramatsu S., Lynn K., Abe A., Kumaraswamy A., Tanimoto H. (2019) "Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors". In: Cell Reports.

General Notes

  1. Confocal dataset of Drosophila brains for GAL4 expression inserted in endogenous receptor genes. Expression is visualized with reporters for plasma membrane and nuclei with counterstaining of neuropils.
  2. Each gene folder contains multiple subfolders with different labellings. These are named "UAS-myr-GFP_UAS-His-RFP", "UAS-myr-GFP_UAS-His-RFP", and "UAS-mCD8-GFP_brp-SNAP_IS2_Registration".

Folder Specific Notes

UAS-myr-GFP_UAS-His-RFP

It contains a few (typically three) full-brain multi-channel TIFF stacks visualized by myristoylated GFP (Pfeiffer et al. Genetics 2010) and His2A::RFP (Pandey et al. J Cell Sci, 2005). Ch1: myr::GFP, Ch2: His2A::RFP.

UAS-mCD8-GFP_brp-SNAP

It contains a few (typically three) central brain multi-TIFF stacks visualized by mCD8::GFP (Pfeiffer et al. Genetics 2010) together with neuropil counterstaining by SNAP-tagged active zone protein Bruchpilot (Kohl et al. PNAS 2014). Ch1: brp::SNAP, Ch2: mCD8::GFP.

UAS-mCD8-GFP_brp-SNAP_IS2_Registration

It contains central brain NRRD stacks derived from registering confocal data in the "UAS-mCD8-GFP_brp-SNAP" folder into the IS2 standard brain (Cachero et al., Curr Biol., 2010) by Computational Morphometry Toolkit (http://hdl.handle.net/10380/3140). "no1", "no2", "no3" in the file name stand for different individuals. "_01": brp-SNAP channel, "_02": GFP channel.

datacite.yml
Title Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors
Authors Kondo,Shu;Genetic Strains Research Center, National Institute of Genetics, Mishima, 411-8540, Japan;ORCID:0000-0002-4625-8379
Takahashi,Takahiro;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan;ORCID:0000-0002-9213-0587
Yamagata,Nobuhiro;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan;ORCID:0000-0003-1993-2038
Imanishi,Yasuhito;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan
Katow,Hidetaka;Genetic Strains Research Center, National Institute of Genetics, Mishima, 411-8540, Japan
Hiramatsu,Shun;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan
Lynn,Katarina B.;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan
Abe,Ayako;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan
Kumaraswamy,Ajayrama;Ludwig-Maximilians-Universität München, Planegg-Martinsried, D-82152, Germany;ORCID:0000-0001-7928-877X
Tanimoto,Hiromu;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan;ORCID:0000-0001-5880-6064
Description This repository contains confocal data of T2A-GAL4 knock-in lines used for investigating the cellular organization and function of neurotransmitter receptor systems in the fly brain.
License Creative Commons Attribution 4.0 International Public License (https://creativecommons.org/licenses/by/4.0/)
References Kondo S., Takahashi T., Yamagata N., Imanishi Y., Katow H., Hiramatsu S., Lynn K., Abe A., Kumaraswamy A., Tanimoto H. (2019) Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors. In: Cell Reports [doi:10.xxx/zzzz] (IsSupplementTo)
Funding MEXT/JSPS KAKENHI, 26840110, 17H04765, 26250001, 17H01378, 25890003, 26120705, 26119503, 15K14307, 16H01496, 17H05545
the Strategic Research Program for Brain Sciences “Bioinformatics for Brain Sciences”
Grant-in-Aid for Scientific Research on Innovative Areas (Comprehensive Brain Science Network)
the Naito Foundation
the Kurata Memorial Hitachi Science and Technology Foundation
the Uehara Memorial Foundation
the Mochida Memorial Foundation for Medical and Pharmaceutical Research
Keywords Neuroscience
Imaging
Neurotransmitter receptor
Drosophila melanogaster
Brain
Resource Type Dataset