We use cookies to ensure you get the best experience on our website
Strona główna Odkrywaj Pomoc News
Zarejestruj się Zaloguj się
doi
/
T2A-GAL4_Library
sklonowany z Tanimoto_lab/T2A-GAL4_Library
DOI
10.12751/g-node.10246f
1
0
Forkuj 0
Pliki

Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors

Drzewo: d27e23de78
Gałęzie Tagi
T2A-GAL4_Lib...
Ajayrama Kumaraswamy d27e23de78 Update 'datacite.yml' 4 lat temu
5-HT1B e9102e0f5d all files uploaded 4 lat temu
5-HT2A e9102e0f5d all files uploaded 4 lat temu
5-HT2B e9102e0f5d all files uploaded 4 lat temu
5-HT7 e9102e0f5d all files uploaded 4 lat temu
AkhR e9102e0f5d all files uploaded 4 lat temu
AstA-R1 e9102e0f5d all files uploaded 4 lat temu
AstA-R2 e9102e0f5d all files uploaded 4 lat temu
AstC-R1 e9102e0f5d all files uploaded 4 lat temu
AstC-R2 e9102e0f5d all files uploaded 4 lat temu
CCAP-R e9102e0f5d all files uploaded 4 lat temu
CCHa1-R e9102e0f5d all files uploaded 4 lat temu
CCHa2-R e9102e0f5d all files uploaded 4 lat temu
CCKLR-17D1 e9102e0f5d all files uploaded 4 lat temu
CCKLR-17D3 e9102e0f5d all files uploaded 4 lat temu
CG12344 e9102e0f5d all files uploaded 4 lat temu
CG13229 e9102e0f5d all files uploaded 4 lat temu
CG13575 e9102e0f5d all files uploaded 4 lat temu
CG13995 e9102e0f5d all files uploaded 4 lat temu
CG32547 e9102e0f5d all files uploaded 4 lat temu
CG33639 e9102e0f5d all files uploaded 4 lat temu
CG43795 e9102e0f5d all files uploaded 4 lat temu
CG7589 e9102e0f5d all files uploaded 4 lat temu
CapaR e9102e0f5d all files uploaded 4 lat temu
CrzR e9102e0f5d all files uploaded 4 lat temu
Dh-31R e9102e0f5d all files uploaded 4 lat temu
Dh44-R2 e9102e0f5d all files uploaded 4 lat temu
Dop1R1 e9102e0f5d all files uploaded 4 lat temu
Dop1R2 e9102e0f5d all files uploaded 4 lat temu
Dop2R e9102e0f5d all files uploaded 4 lat temu
DopEcR e9102e0f5d all files uploaded 4 lat temu
GABA-B-R1 e9102e0f5d all files uploaded 4 lat temu
GABA-B-R2 e9102e0f5d all files uploaded 4 lat temu
GluCLα e9102e0f5d all files uploaded 4 lat temu
GluRIB e9102e0f5d all files uploaded 4 lat temu
GluRIIA e9102e0f5d all files uploaded 4 lat temu
GluRIIB e9102e0f5d all files uploaded 4 lat temu
GluRIIC e9102e0f5d all files uploaded 4 lat temu
GluRIID e9102e0f5d all files uploaded 4 lat temu
GluRIIE e9102e0f5d all files uploaded 4 lat temu
Grd e9102e0f5d all files uploaded 4 lat temu
Gyc76C e704f595bc reorganized existing tiff files into folder UAS-myr-GFP_UAS-His-RFP 4 lat temu
KaiR1D e9102e0f5d all files uploaded 4 lat temu
Lcch3 e9102e0f5d all files uploaded 4 lat temu
Lgr1 e9102e0f5d all files uploaded 4 lat temu
Lgr3 e9102e0f5d all files uploaded 4 lat temu
Lgr4 e9102e0f5d all files uploaded 4 lat temu
NPFR e9102e0f5d all files uploaded 4 lat temu
Nmdar1 e9102e0f5d all files uploaded 4 lat temu
Nmdar2 e9102e0f5d all files uploaded 4 lat temu
Oamb e9102e0f5d all files uploaded 4 lat temu
Octβ2R e9102e0f5d all files uploaded 4 lat temu
Octβ3R e9102e0f5d all files uploaded 4 lat temu
PK1-R e9102e0f5d all files uploaded 4 lat temu
PK2-R2 e9102e0f5d all files uploaded 4 lat temu
Pdfr e9102e0f5d all files uploaded 4 lat temu
RYa-R e9102e0f5d all files uploaded 4 lat temu
SIFaR e9102e0f5d all files uploaded 4 lat temu
SPR e9102e0f5d all files uploaded 4 lat temu
TkR86C e9102e0f5d all files uploaded 4 lat temu
TyrRII e9102e0f5d all files uploaded 4 lat temu
hec e9102e0f5d all files uploaded 4 lat temu
mAChR-A e9102e0f5d all files uploaded 4 lat temu
mAChR-B e9102e0f5d all files uploaded 4 lat temu
mGluR e9102e0f5d all files uploaded 4 lat temu
mtt e9102e0f5d all files uploaded 4 lat temu
nAChRα1 e9102e0f5d all files uploaded 4 lat temu
nAChRα2 e9102e0f5d all files uploaded 4 lat temu
nAChRα3 e9102e0f5d all files uploaded 4 lat temu
nAChRα6 e9102e0f5d all files uploaded 4 lat temu
nAChRα7 e9102e0f5d all files uploaded 4 lat temu
nAChRβ1 e9102e0f5d all files uploaded 4 lat temu
nAChRβ2 e9102e0f5d all files uploaded 4 lat temu
nAChRβ3 e9102e0f5d all files uploaded 4 lat temu
ort e9102e0f5d all files uploaded 4 lat temu
rk e9102e0f5d all files uploaded 4 lat temu
LICENSE 6a478a8636 Initial commit 5 lat temu
README.md 285014d3af Update 'README.md' 4 lat temu
datacite.yml d27e23de78 Update 'datacite.yml' 4 lat temu

README.md

Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors

This repository contains confocal data of T2A-GAL4 knock-in lines used for investigating the cellular organization and function of neurotransmitter receptor systems in the fly brain. Flies and experimental conditions should be referred to:

Kondo S., Takahashi T., Yamagata N., Imanishi Y., Katow H., Hiramatsu S., Lynn K., Abe A., Kumaraswamy A., Tanimoto H. (2019) "Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors". In: Cell Reports.

General Notes

  1. Confocal dataset of Drosophila brains for GAL4 expression inserted in endogenous receptor genes. Expression is visualized with reporters for plasma membrane and nuclei with counterstaining of neuropils.
  2. Each gene folder contains multiple subfolders with different labellings. These are named "UAS-myr-GFP_UAS-His-RFP", "UAS-myr-GFP_UAS-His-RFP", and "UAS-mCD8-GFP_brp-SNAP_IS2_Registration".

Folder Specific Notes

UAS-myr-GFP_UAS-His-RFP

It contains a few (typically three) full-brain multi-channel TIFF stacks visualized by myristoylated GFP (Pfeiffer et al. Genetics 2010) and His2A::RFP (Pandey et al. J Cell Sci, 2005). Ch1: myr::GFP, Ch2: His2A::RFP.

UAS-mCD8-GFP_brp-SNAP

It contains a few (typically three) central brain multi-TIFF stacks visualized by mCD8::GFP (Pfeiffer et al. Genetics 2010) together with neuropil counterstaining by SNAP-tagged active zone protein Bruchpilot (Kohl et al. PNAS 2014). Ch1: brp::SNAP, Ch2: mCD8::GFP.

UAS-mCD8-GFP_brp-SNAP_IS2_Registration

It contains central brain NRRD stacks derived from registering confocal data in the "UAS-mCD8-GFP_brp-SNAP" folder into the IS2 standard brain (Cachero et al., Curr Biol., 2010) by Computational Morphometry Toolkit (http://hdl.handle.net/10380/3140). "no1", "no2", "no3" in the file name stand for different individuals. "_01": brp-SNAP channel, "_02": GFP channel.

datacite.yml
Title Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors
Authors Kondo,Shu;Genetic Strains Research Center, National Institute of Genetics, Mishima, 411-8540, Japan;ORCID:0000-0002-4625-8379
Takahashi,Takahiro;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan;ORCID:0000-0002-9213-0587
Yamagata,Nobuhiro;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan;ORCID:0000-0003-1993-2038
Imanishi,Yasuhito;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan
Katow,Hidetaka;Genetic Strains Research Center, National Institute of Genetics, Mishima, 411-8540, Japan
Hiramatsu,Shun;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan
Lynn,Katarina B.;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan
Abe,Ayako;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan
Kumaraswamy,Ajayrama;Ludwig-Maximilians-Universität München, Planegg-Martinsried, D-82152, Germany;ORCID:0000-0001-7928-877X
Tanimoto,Hiromu;Tohoku University Graduate School of Life Sciences, Sendai, 980-8577, Japan;ORCID:0000-0001-5880-6064
Description This repository contains confocal data of T2A-GAL4 knock-in lines used for investigating the cellular organization and function of neurotransmitter receptor systems in the fly brain.
License Creative Commons Attribution 4.0 International Public License (https://creativecommons.org/licenses/by/4.0/)
References Kondo S., Takahashi T., Yamagata N., Imanishi Y., Katow H., Hiramatsu S., Lynn K., Abe A., Kumaraswamy A., Tanimoto H. (2019) Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors. In: Cell Reports [doi:10.xxx/zzzz] (IsSupplementTo)
Funding MEXT/JSPS KAKENHI, 26840110, 17H04765, 26250001, 17H01378, 25890003, 26120705, 26119503, 15K14307, 16H01496, 17H05545
the Strategic Research Program for Brain Sciences “Bioinformatics for Brain Sciences”
Grant-in-Aid for Scientific Research on Innovative Areas (Comprehensive Brain Science Network)
the Naito Foundation
the Kurata Memorial Hitachi Science and Technology Foundation
the Uehara Memorial Foundation
the Mochida Memorial Foundation for Medical and Pharmaceutical Research
Keywords Neuroscience
Imaging
Neurotransmitter receptor
Drosophila melanogaster
Brain
Resource Type Dataset