Locomotion of Drosophila melanogaster larvae in the absence of any experimental stimuli
Summary
Groups of ~ 20 naive Drosophila melanogaster larvae (Canton S) were placed on agarose-filled Petri dishes of 15 cm diameter with no particular stimuli. Their behaviour was recorded from above for 3 min with 16 frames/s and tracked as described in Paisios et al. 2017. This data set contains # experiments and approx. # animals in total. For each experiment, it features a video with the recorded larvae, standardized meta data and individual csv files for each tracked animal. The provided data can be used to analyze basic locomotion and behaviour of Drosophila larvae, and to benchmark video-analysis tools.
Structure
Each individual experiment is saved in a separate folder with standardized names featuring the internal name of the used setup, the date and the time of the experiment. In the folder, individual csv files feature the information of tracked animals. The coloums from left to right feature the video frame; the x and y coordinates of 12 spine points from rear to front; the x and y coordinates of 22 contour points; the length, width, perimeter, area and average gray value of the larva; #. Coordinates are in mm, with the center of the video set to 0,0. Length, width, perimeter and area are given in pixel. Each experiment has a subfolder that contains the annotated video of the experiment as well as a meta data file that gives some basic information of the experiment.
Related publications
Thane M, Viswanathan V, Meyer TC, Paisios E, Schleyer M. 2019. Modulations of microbehaviour by associative memory strength in Drosophila larvae. PloS One 14: e0224154. https://doi.org/10.1371/journal.pone.0224154
Paisios E, Rjosk A, Pamir E, Schleyer M. 2017. Common microbehavioural 'footprint' of two distinct classes of conditioned aversion. Learn Mem 24:191-198. https://doi.org/10.1101/lm.045062.117
