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- function BehavStats=all_behavior(RAWblocks, RAWCued, plot_region, plot_sub_figures, perform_stats, Colors)
- %% interspersed vs blocks
- %subselect sessions of interest
- if strcmp(plot_region,'Both')
- RAW=RAWblocks;
- else
-
- for i=1:length(RAWblocks)
- name=char(RAWblocks(i).Region);
- region{i,1}=name(1:2);
- end
- region_sessions = strcmp(plot_region,region);
- RAW=RAWblocks(region_sessions);
- end
- % Latency
- % Find the indicies of all blocks and interspersed sessions
- blocked_sessions = strcmp('B',{RAW.Blocks})';
- interspersed_sessions = strcmp('I',{RAW.Blocks})';
- num_sessions = length(RAW);
- num_trials = 60;
- % preallocate arrays
- latencyR1 = nan(num_sessions, num_trials);
- latencyR2 = nan(num_sessions, num_trials);
- fraction_completed = zeros(num_sessions,2); %suc,malt
- rat_name = cell(num_sessions,1);
- for session = 1:num_sessions
-
- % Make logical index vectors to call every event type
- PER1 = strcmp('PER1',RAW(session).Einfo(:,2)); %finds the index of the event
- PER2 = strcmp('PER2',RAW(session).Einfo(:,2)); %finds the index of the event
- CueR1 = strcmp('CueR1',RAW(session).Einfo(:,2)); %finds the index of the event
- CueR2 = strcmp('CueR2',RAW(session).Einfo(:,2)); %finds the index of the event
- PECue = strcmp('PECue',RAW(session).Einfo(:,2)); %finds the index of the event
-
- % Latency Stuff
- suc_latencies = cell2mat(MakePSR04(RAW(session).Erast(PECue),RAW(session).Erast{CueR1},[0 20],{2,'first'}));
- malt_latencies = cell2mat(MakePSR04(RAW(session).Erast(PECue),RAW(session).Erast{CueR2},[0 20],{2,'first'}));
- latencyR1(session,1:length(suc_latencies)) = suc_latencies;
- latencyR2(session,1:length(malt_latencies)) = malt_latencies;
-
- % Trial Completion Stuff
- suc_completed = length(RAW(session).Erast{PER1,1});
- suc_total = length(RAW(session).Erast{CueR1,1});
- malt_completed = length(RAW(session).Erast{PER2,1});
- malt_total = length(RAW(session).Erast{CueR2,1});
- fraction_completed(session,:) = [suc_completed/suc_total,malt_completed/malt_total];
-
- % Rat names
- name=char(RAW(session).Ninfo(1,1));
- rat_name{session,1} = name(1:3);
- end
- % Latency stuff
- median_latencyR1 = median(latencyR1,2,'omitnan');
- median_latencyR2 = median(latencyR2,2,'omitnan');
- % Average for rat
- unique_rat_names = unique(rat_name);
- rat_latency_data = zeros(length(unique_rat_names),4);
- rat_completion_data = zeros(length(unique_rat_names),4);
- rat_latency_data_sem = zeros(length(unique_rat_names),4);
- rat_completion_data_sem = zeros(length(unique_rat_names),4);
- for rat = 1:length(unique_rat_names)
- rat_index = strcmp(unique_rat_names{rat}, rat_name);
- rat_latency_data(rat,:) = [mean(median_latencyR1(interspersed_sessions & rat_index)),... % Sucrose inter
- mean(median_latencyR2(interspersed_sessions & rat_index)),... % Malto inter
- mean(median_latencyR1(blocked_sessions & rat_index)),... % Sucrose block
- mean(median_latencyR2(blocked_sessions & rat_index))]; % Malto block
- rat_completion_data(rat,:) = [mean(fraction_completed(interspersed_sessions & rat_index, 1)),... % Sucrose inter
- mean(fraction_completed(interspersed_sessions & rat_index, 2)),... % Malto inter
- mean(fraction_completed(blocked_sessions & rat_index, 1)),... % Sucrose block
- mean(fraction_completed(blocked_sessions & rat_index, 2))]; % Malto block
- rat_latency_data_sem(rat,:) = [nanste(median_latencyR1(interspersed_sessions & rat_index),1),... % Sucrose inter
- nanste(median_latencyR2(interspersed_sessions & rat_index),1),... % Malto inter
- nanste(median_latencyR1(blocked_sessions & rat_index),1),... % Sucrose block
- nanste(median_latencyR2(blocked_sessions & rat_index),1)]; % Malto block
- rat_completion_data_sem(rat,:) = [nanste(fraction_completed(interspersed_sessions & rat_index, 1),1),... % Sucrose inter
- nanste(fraction_completed(interspersed_sessions & rat_index, 2),1),... % Malto inter
- nanste(fraction_completed(blocked_sessions & rat_index, 1),1),... % Sucrose block
- nanste(fraction_completed(blocked_sessions & rat_index, 2),1)]; % Malto block
- end
- mean_latency_data = [mean(median_latencyR1(interspersed_sessions)),... % Sucrose inter
- mean(median_latencyR2(interspersed_sessions)),... % Malto inter
- mean(median_latencyR1(blocked_sessions)),... % Sucrose block
- mean(median_latencyR2(blocked_sessions));... % Malto block
- nanste(median_latencyR1(interspersed_sessions),1),... % Sucrose inter
- nanste(median_latencyR2(interspersed_sessions),1),... % Malto inter
- nanste(median_latencyR1(blocked_sessions),1),... % Sucrose block
- nanste(median_latencyR2(blocked_sessions),1)]; % Malto block
- mean_completion_data = [mean(fraction_completed(interspersed_sessions, 1)),... % Sucrose inter
- mean(fraction_completed(interspersed_sessions, 2)),... % Malto inter
- mean(fraction_completed(blocked_sessions, 1)),... % Sucrose block
- mean(fraction_completed(blocked_sessions, 2));... % Malto block
- nanste(fraction_completed(interspersed_sessions, 1),1),... % Sucrose inter
- nanste(fraction_completed(interspersed_sessions, 2),1),... % Malto inter
- nanste(fraction_completed(blocked_sessions, 1),1),... % Sucrose block
- nanste(fraction_completed(blocked_sessions, 2),1)]; % Malto block
- % make figure
- figure('Units','inches','Position',[3,3,8,5]);
- % Latency subplot
- mean_data = {mean_latency_data, mean_completion_data};
- rat_data = {rat_latency_data, rat_completion_data, rat_latency_data_sem, rat_completion_data_sem};
- color = {Colors('sucrose'),Colors('maltodextrin'),Colors('blockrose'),Colors('blockrodextrin')};
- titles = {'Latency','Completion'};
- for plot_type = 1:2
- subplot(3,4,[5+(plot_type-1)*2 6+(plot_type-1)*2])
- hold on
- for rat = 1:length(rat_latency_data)
- xvals=[0.9 1.9]+(rat-1)*0.2/(length(unique_rat_names)-1);
- errorbar(xvals,rat_data{plot_type}(rat,1:2),rat_data{plot_type+2}(rat,1:2),'Color',[.7 .7 .7])
- errorbar(xvals+2,rat_data{plot_type}(rat,3:4),rat_data{plot_type+2}(rat,3:4),'Color',[.7 .7 .7])
- end
- for i = 1:4
- errorbar(i,mean_data{plot_type}(1,i),mean_data{plot_type}(2,i),'o','Color',color{i},'MarkerFaceColor',color{i})
- end
- if plot_type == 1
- % plot([1,2],[5,5],'k')
- % plot([3,4],[4.5,4.5],'k')
- % plot([1.5,3.5],[5.5,5.5],'k')
- % plot([1.5,1.5],[5.5,5],'k')
- % plot([3.5,3.5],[5.5,4.5],'k')
- % text(2.5, 5.7, '*','Color','k','FontSize',16)
- % text(2.98, 1.77, '#','Color','k') %This is to note that the two sucrose latencies are significantly different, which should be explained in the figure legend.
- axis([0.5 9.5 0 10]);
- ylabel('Latency (sec)');
- else
- axis([0.5 9.5 0 1]);
- ylabel('Fraction of trials completed')
- end
- end
- subplot(3,4,1:3)
- sessions_to_use = [1 8];
- % figure;
- session_end = strcmp('MedEnd',RAW(sessions_to_use(1)).Einfo(:,2)); %finds the index of the event
- stop(1) = RAW(sessions_to_use(1)).Erast{session_end,1};
- session_end = strcmp('MedEnd',RAW(sessions_to_use(2)).Einfo(:,2)); %finds the index of the event
- stop(2) = RAW(sessions_to_use(2)).Erast{session_end,1};
- line([0,1+stop(1)/60],[4.3,4.3],'Color','k')
- line([0,1+stop(2)/60],[6.3,6.3],'Color','k')
- for session = 1:length(sessions_to_use)
-
- %get events for this session
- RD1 = strcmp('RD1',RAW(sessions_to_use(session)).Einfo(:,2)); %finds the index of the event
- RD2 = strcmp('RD2',RAW(sessions_to_use(session)).Einfo(:,2)); %finds the index of the event
-
- suc_delivery = RAW(sessions_to_use(session)).Erast{RD1,1}/60;
- malt_delivery = RAW(sessions_to_use(session)).Erast{RD2,1}/60;
- for trial = 1:length(suc_delivery)
- line([suc_delivery(trial),suc_delivery(trial)],[3+2*(session-1)+1.1,3+2*(session-1)+1.7],'Color',color{3-2*(session-1)})
- end
- for trial = 1:length(malt_delivery)
- line([malt_delivery(trial),malt_delivery(trial)],[3+2*(session-1)+0.9,3+2*(session-1)+1.5],'Color',color{4-2*(session-1)})
- end
- end
- % title('Two Example Sessions','FontSize',11)
- if perform_stats
- % Latency
- suc_interspersed = median_latencyR1(interspersed_sessions);
- malt_interspersed = median_latencyR2(interspersed_sessions);
- suc_blocked = median_latencyR1(blocked_sessions);
- malt_blocked = median_latencyR2(blocked_sessions);
-
- num_interspersed = length(suc_interspersed);
- num_blocked = length(suc_blocked);
- rat_interspersed = rat_name(interspersed_sessions);
- rat_blocked = rat_name(blocked_sessions);
-
- % t=table(cat(1,rat_interspersed,rat_blocked),categorical(cat(1,zeros(num_interspersed,1),ones(num_blocked,1))),cat(1,suc_interspersed,suc_blocked),cat(1,malt_interspersed,malt_blocked),'variablenames',{'subject','blocked','sucrose','maltodextrin'});
- % vars=table({'Suc'; 'Mal'},'variablenames',{'Reward'});
- % rm=fitrm(t,'sucrose-maltodextrin~subject+blocked','WithinDesign',vars);
- % BehavStats.IntblockLatency.ranovatbl=ranova(rm);
- % BehavStats.IntblockLatency.blocked=multcompare(rm,'blocked');
- % BehavStats.IntblockLatency.reward=multcompare(rm,'Reward');
- % BehavStats.IntblockLatency.blockedbyreward=multcompare(rm,'blocked','by','Reward');
-
-
- % figure;
- [~,BehavStats.IntblockLatency.anovatbl,stats] = anovan(cat(1,suc_interspersed,malt_interspersed,suc_blocked,malt_blocked),...
- {cat(1,zeros(num_interspersed*2,1),ones(num_blocked*2,1)),...
- cat(1,zeros(num_interspersed,1),ones(num_interspersed,1),zeros(num_blocked,1),ones(num_blocked,1)),...
- cat(1,rat_interspersed,rat_interspersed,rat_blocked,rat_blocked)},...
- 'varnames',{'Int/Block','Reward','Subject'},'model',[1 0 0;1 1 0;0 0 1],... %[1 0 0;0 1 0;1 1 0;0 0 1],...
- 'random',3,'display','off');
- [BehavStats.IntblockLatency.multcompare] = multcompare(stats,'dimension',[1 2],'display','off');
- BehavStats.IntblockLatency.stats=stats;
-
- % Trials completed
- suc_inter = fraction_completed(interspersed_sessions,1);
- malt_inter = fraction_completed(interspersed_sessions,2);
- suc_block = fraction_completed(blocked_sessions,1);
- malt_block = fraction_completed(blocked_sessions,2);
-
- % t=table(cat(1,rat_interspersed,rat_blocked),categorical(cat(1,zeros(num_interspersed,1),ones(num_blocked,1))),cat(1,suc_inter,suc_block),cat(1,malt_inter,malt_block),'variablenames',{'subject','blocked','sucrose','maltodextrin'});
- % vars=table({'Suc'; 'Mal'},'variablenames',{'Reward'});
- % rm=fitrm(t,'sucrose-maltodextrin~subject+blocked','WithinDesign',vars);
- % BehavStats.IntblockCompletion.ranovatbl=ranova(rm);
- % BehavStats.IntblockCompletion.blocked=multcompare(rm,'blocked');
- % BehavStats.IntblockCompletion.reward=multcompare(rm,'Reward');
- % BehavStats.IntblockCompletion.blockedbyreward=multcompare(rm,'blocked','by','Reward');
-
- % figure;
- [~,BehavStats.IntblockCompletion.anovatbl,stats] = anovan(cat(1,suc_inter,malt_inter,suc_block,malt_block),...
- {cat(1,zeros(num_interspersed*2,1),ones(num_blocked*2,1)),...
- cat(1,zeros(num_interspersed,1),ones(num_interspersed,1),zeros(num_blocked,1),ones(num_blocked,1)),...
- cat(1,rat_interspersed,rat_interspersed,rat_blocked,rat_blocked)},...
- 'varnames',{'Int/Block','Reward','Subject'},'model',[1 0 0;1 1 0;0 0 1],... %[1 0 0;0 1 0;1 1 0;0 0 1],...
- 'random',3,'display','off');
- [BehavStats.IntblockCompletion.multcompare] = multcompare(stats,'dimension',[1 2],'display','off');
- BehavStats.IntblockCompletion.stats=stats;
- end
- %lick plots
- global Dura Tm BSIZE %Tbin
- BSIZE=0.05; %what's the bin for the PSTH?
- Dura=[-4 15]; Tm=Dura(1):BSIZE:Dura(2);
- %Tbin=-0.5:0.005:0.5; %window used to determine the optimal binsize
- %Smoothing PSTHs
- %smoothing filter
- smoothbins=25; %number of previous bins used to smooth
- halfnormal=makedist('HalfNormal','mu',0,'sigma',8); %std=3.98
- filterweights=pdf(halfnormal,[0:smoothbins]);
- %generate lick PSTHs
- for session = 1:num_sessions
-
- % Make logical index vectors to call every event type
- R1 = strcmp('RD1',RAW(session).Einfo(:,2)); %finds the index of the event
- R2 = strcmp('RD2',RAW(session).Einfo(:,2)); %finds the index of the event
- Licks = strcmp('Licks',RAW(session).Einfo(:,2)); %finds the index of the event
-
- EvList{1}=RAW(session).Erast{R1};
- EvList{2}=RAW(session).Erast{R2};
- for k=1:2
- [PSR1,N1]=MakePSR04(RAW(session).Erast(Licks),EvList{k},Dura,{1});% makes collpased rasters. PSR1 is a cell(neurons)
- [PTH1,~,~]=MakePTH07(PSR1,repmat(N1,size(RAW(session).Erast(Licks),1),1),{2,0,BSIZE});%-----Fixed bin used here
- PTH1smooth=[];
- for l=1:length(Tm)
- PTH1smooth(1,l)=sum(PTH1(1,l-min([l-1 smoothbins]):l).*fliplr(filterweights(1:min([l smoothbins+1]))))/sum(filterweights(1:min([l smoothbins+1])));
- end
- Lick.IntBlock(k).PSTHraw(session,:)=PTH1smooth;
- end
- end
- %plotting
- Xaxis=[-2 12];
- Ishow=find(Tm>=Xaxis(1) & Tm<=Xaxis(2));
- time1=Tm(Ishow);
- for type=1:2
-
- if type==1 Sel=interspersed_sessions; end
- if type==2 Sel=blocked_sessions; end
-
- psth1=nanmean(Lick.IntBlock(1).PSTHraw(Sel,Ishow),1);
- sem1=nanste(Lick.IntBlock(1).PSTHraw(Sel,Ishow),1); %calculate standard error of the mean
- up1=psth1+sem1;
- down1=psth1-sem1;
- psthE=nanmean(Lick.IntBlock(2).PSTHraw(Sel,Ishow),1);
- semE=nanste(Lick.IntBlock(2).PSTHraw(Sel,Ishow),1); %calculate standard error of the mean
- upE=psthE+semE;
- downE=psthE-semE;
- %plotting
- subplot(4,4,12+type);
- hold on;
- plot(time1,psth1,'Color',color{1+(type-1)*2},'linewidth',1);
- plot(time1,psthE,'Color',color{2+(type-1)*2},'linewidth',1);
- patch([time1,time1(end:-1:1)],[up1,down1(end:-1:1)],color{1+(type-1)*2},'EdgeColor','none');alpha(0.5);
- patch([time1,time1(end:-1:1)],[upE,downE(end:-1:1)],color{2+(type-1)*2},'EdgeColor','none');alpha(0.5);
- plot([0 0],[-2 8],':','color','k','linewidth',0.75);
- axis([Xaxis(1) Xaxis(2) 0 8]);
- xlabel('Seconds from reward delivery');
- if type==1 ylabel('Mean lick rate (licks/s)'); end
- if type==1 title('Interspersed'); end
- if type==2 title('Blocked'); end
- legend('Suc trials','Mal trials');
- end
- %% cued sessions
- %subselect sessions of interest
- for i=1:length(RAWCued)
- name=char(RAWCued(i).Ninfo(1,1));
- sessrat(i,1)=str2num(name(2:3));
- sessday(i,1)=str2num(name(8:9));
- end
- %decide which sessions to include
- inc_sessions = (sessrat==3 | sessrat==4 | sessrat==9 | sessrat==10) & sessday>10;
- RAW=RAWCued(inc_sessions);
- %RAW=RAWCued; %all sessions
- % Latency
- % Find the indicies of all blocks and interspersed sessions
- num_sessions = length(RAW);
- num_trials = 60; %just for pre-allocating
- % preallocate arrays
- latencyNo1 = nan(num_sessions, num_trials); %non-predictive sucrose
- latencyNo2 = nan(num_sessions, num_trials); %non-predictive maltodextrin
- latencyPr1 = nan(num_sessions, num_trials); %predictive sucrose
- latencyPr2 = nan(num_sessions, num_trials); %predictive maltodextrin
- fraction_completed = zeros(num_sessions,4); %Predictive suc, pm, ns, nm
- rat_name = cell(num_sessions,1);
- for session = 1:num_sessions
-
- % Make logical index vectors to call every event type
- PENo1 = strcmp('PECue31',RAW(session).Einfo(:,2)); %finds the index of the event
- PENo2 = strcmp('PECue32',RAW(session).Einfo(:,2)); %finds the index of the event
- PEPr1 = strcmp('PECue1',RAW(session).Einfo(:,2)); %finds the index of the event
- PEPr2 = strcmp('PECue2',RAW(session).Einfo(:,2)); %finds the index of the event
- Cue1 = strcmp('Cue1',RAW(session).Einfo(:,2)); %finds the index of the event
- Cue2 = strcmp('Cue2',RAW(session).Einfo(:,2)); %finds the index of the event
- Cue31 = strcmp('Cue31',RAW(session).Einfo(:,2)); %finds the index of the event
- Cue32 = strcmp('Cue32',RAW(session).Einfo(:,2)); %finds the index of the event
- PECue = strcmp('PECue',RAW(session).Einfo(:,2)); %finds the index of the event
-
- % Latency Stuff
- No1_latencies = cell2mat(MakePSR04(RAW(session).Erast(PECue),RAW(session).Erast{Cue31},[0 20],{2,'first'}));
- latencyNo1(session,1:length(No1_latencies)) = No1_latencies;
- No2_latencies = cell2mat(MakePSR04(RAW(session).Erast(PECue),RAW(session).Erast{Cue32},[0 20],{2,'first'}));
- latencyNo2(session,1:length(No2_latencies)) = No2_latencies;
-
- Pr1_latencies = cell2mat(MakePSR04(RAW(session).Erast(PECue),RAW(session).Erast{Cue1},[0 20],{2,'first'}));
- latencyPr1(session,1:length(Pr1_latencies)) = Pr1_latencies;
- Pr2_latencies = cell2mat(MakePSR04(RAW(session).Erast(PECue),RAW(session).Erast{Cue2},[0 20],{2,'first'}));
- latencyPr2(session,1:length(Pr2_latencies)) = Pr2_latencies;
-
- % Trial Completion Stuff
- No1_completed = length(RAW(session).Erast{PENo1,1});
- No1_total = length(RAW(session).Erast{Cue31,1});
- No2_completed = length(RAW(session).Erast{PENo2,1});
- No2_total = length(RAW(session).Erast{Cue32,1});
- Pr1_completed = length(RAW(session).Erast{PEPr1,1});
- Pr1_total = length(RAW(session).Erast{Cue1,1});
- Pr2_completed = length(RAW(session).Erast{PEPr2,1});
- Pr2_total = length(RAW(session).Erast{Cue2,1});
- fraction_completed(session,:) = [No1_completed/No1_total,No2_completed/No2_total,Pr1_completed/Pr1_total,Pr2_completed/Pr2_total];
-
- % Rat names
- name=char(RAW(session).Ninfo(1,1));
- rat_name{session,1} = name(1:3);
- end
- % Latency stuff
- median_latencyNo1 = median(latencyNo1,2,'omitnan');
- median_latencyNo2 = median(latencyNo2,2,'omitnan');
- median_latencyPr1 = median(latencyPr1,2,'omitnan');
- median_latencyPr2 = median(latencyPr2,2,'omitnan');
- % Average for rat
- unique_rat_names = unique(rat_name);
- rat_latency_data = zeros(length(unique_rat_names),4);
- rat_completion_data = zeros(length(unique_rat_names),4);
- rat_latency_data_sem = zeros(length(unique_rat_names),4);
- rat_completion_data_sem = zeros(length(unique_rat_names),4);
- for rat = 1:length(unique_rat_names)
- rat_index = strcmp(unique_rat_names{rat}, rat_name);
- rat_latency_data(rat,:) = [mean(median_latencyNo1(rat_index)),... % Sucrose non-pr
- mean(median_latencyNo2(rat_index)),... % Malto non-pr
- mean(median_latencyPr1(rat_index)),... % Sucrose pr
- mean(median_latencyPr2(rat_index))]; % Malto pr
- rat_completion_data(rat,:) = [mean(fraction_completed(rat_index, 1)),... % Sucrose non-pr
- mean(fraction_completed(rat_index, 2)),... % Malto non-pr
- mean(fraction_completed(rat_index, 3)),... % Sucrose pr
- mean(fraction_completed(rat_index, 4))]; % Malto pr
- rat_latency_data_sem(rat,:) = [nanste(median_latencyNo1(rat_index),1),... % Sucrose non-pr
- nanste(median_latencyNo2(rat_index),1),... % Malto non-pr
- nanste(median_latencyPr1(rat_index),1),... % Sucrose pr
- nanste(median_latencyPr2(rat_index),1)]; % Malto pr
- rat_completion_data_sem(rat,:) = [nanste(fraction_completed(rat_index, 1),1),...
- nanste(fraction_completed(rat_index, 2),1),...
- nanste(fraction_completed(rat_index, 3),1),...
- nanste(fraction_completed(rat_index, 4),1)];
- end
- mean_latency_data = [mean(median_latencyNo1),... % Sucrose non-pr
- mean(median_latencyNo2),... % Malto non-pr
- mean(median_latencyPr1),... % Sucrose pr
- mean(median_latencyPr2);... % Malto pr
- nanste(median_latencyNo1,1),...
- nanste(median_latencyNo2,1),...
- nanste(median_latencyPr1,1),...
- nanste(median_latencyPr2,1)];
- mean_completion_data = [mean(fraction_completed(:, 1)),...
- mean(fraction_completed(:, 2)),...
- mean(fraction_completed(:, 3)),...
- mean(fraction_completed(:, 4));...
- nanste(fraction_completed(:, 1),1),...
- nanste(fraction_completed(:, 2),1),...
- nanste(fraction_completed(:, 3),1),...
- nanste(fraction_completed(:, 4),1)];
- % Latency subplot
- mean_data = {mean_latency_data, mean_completion_data};
- rat_data = {rat_latency_data, rat_completion_data, rat_latency_data_sem, rat_completion_data_sem};
- color = {Colors('sucrose'),Colors('maltodextrin'),Colors('blockrose'),Colors('blockrodextrin')};
- titles = {'Latency','Completion'};
- for plot_type = 1:2
- subplot(3,4,[5+(plot_type-1)*2 6+(plot_type-1)*2])
- hold on
- for rat = 1:length(rat_latency_data)
- xvals=[5.9 6.9]+(rat-1)*0.2/(length(unique_rat_names)-1);
- errorbar(xvals,rat_data{plot_type}(rat,1:2),rat_data{plot_type+2}(rat,1:2),'Color',[.7 .7 .7])
- errorbar(xvals+2,rat_data{plot_type}(rat,3:4),rat_data{plot_type+2}(rat,3:4),'Color',[.7 .7 .7])
- end
- for i = 1:4
- errorbar(i+5,mean_data{plot_type}(1,i),mean_data{plot_type}(2,i),'o','Color',color{i},'MarkerFaceColor',color{i})
- end
- if plot_type == 1
- % plot([6,7],[5,5],'k')
- % plot([8,9],[4.5,4.5],'k')
- % plot([6.5,8.5],[5.5,5.5],'k')
- % plot([6.5,6.5],[5.5,5],'k')
- % plot([8.5,8.5],[5.5,4.5],'k')
- % text(7.5, 5.7, '*','Color','k','FontSize',16)
- % text(2.98, 1.77, '#','Color','k') %This is to note that the two sucrose latencies are significantly different, which should be explained in the figure legend.
- axis([0.5 9.5 0 10]);
- ylabel('Port entry latency (sec)');
- text(0.5,11,'Interspersed');
- text(3,11,'Blocked');
- text(5.5,11,'Non-predictive');
- text(8,11,'Predictive');
- else
- axis([0.5 9.5 0 1]);
- ylabel('Fraction of trials completed')
- text(0.5,1.1,'Interspersed');
- text(3,1.1,'Blocked');
- text(5.5,1.1,'Non-predictive');
- text(8,1.1,'Predictive');
- end
- conditions = {"Suc","Mal","Suc","Mal","Suc","Mal","Suc","Mal"};
- set(gca,'XTick',[1:4 6:9],'XTickLabel',conditions)
-
- end
- subplot(3,4,1:3)
- session_to_use = 2;
- session_end = strcmp('MedEnd',RAW(session_to_use(1)).Einfo(:,2)); %finds the index of the event
- stop = RAW(session_to_use(1)).Erast{session_end,1};
- line([0,1+stop(1)/60],[2.3,2.3],'Color','k')
-
- %get events for this session
- Cue1RD = strcmp('Cue1RD',RAW(session_to_use).Einfo(:,2)); %finds the index of the event
- Cue2RD = strcmp('Cue2RD',RAW(session_to_use).Einfo(:,2)); %finds the index of the event
- Cue3RD1 = strcmp('Cue3RD1',RAW(session_to_use).Einfo(:,2)); %finds the index of the event
- Cue3RD2 = strcmp('Cue3RD2',RAW(session_to_use).Einfo(:,2)); %finds the index of the event
- suc_deliveryN = RAW(session_to_use).Erast{Cue3RD1,1}/60;
- malt_deliveryN = RAW(session_to_use).Erast{Cue3RD2,1}/60;
- suc_deliveryP = RAW(session_to_use).Erast{Cue1RD,1}/60;
- malt_deliveryP = RAW(session_to_use).Erast{Cue2RD,1}/60;
- for trial = 1:length(suc_deliveryN)
- a=line([suc_deliveryN(trial),suc_deliveryN(trial)],[2.1,2.7],'Color',color{1});
- end
- for trial = 1:length(malt_deliveryN)
- b=line([malt_deliveryN(trial),malt_deliveryN(trial)],[1.9,2.5],'Color',color{2});
- end
- for trial = 1:length(suc_deliveryP)
- c=line([suc_deliveryP(trial),suc_deliveryP(trial)],[2.1,2.7],'Color',color{3});
- end
- for trial = 1:length(malt_deliveryP)
- d=line([malt_deliveryP(trial),malt_deliveryP(trial)],[1.9,2.5],'Color',color{4});
- end
- text(60,6.3,'Interspersed');
- text(60,4.3,'Blocked');
- text(105,2.3,'Cued');
- text(0,3,'Sucrose, unpredictable','Color',color{1});
- text(30,3,'Sucrose, predicted by cue','Color',color{3});
- text(0,1.6,'Maltodextrin, unpredictable','Color',color{2});
- text(30,1.6,'Maltodextrin, predicted by cue','Color',color{4});
- XTick=[0 20 40 60 80 100];
- axis([0 110 0.9 7]);
- ax1 = gca;
- ax1.YAxis.Visible = 'off';
- xlabel('Session time (minutes)')
- if perform_stats
-
- % Latency
-
- % t=table(rat_name,median_latencyNo1,median_latencyNo2,median_latencyPr1,median_latencyPr2,'variablenames',{'subject','NPSuc','NPMal','PrSuc','PrMal'});
- % vars=table({'NPSuc';'NPMal';'PrSuc';'PrMal'},{'Non'; 'Non'; 'Pr' ;'Pr'},{'Suc'; 'Mal' ;'Suc' ;'Mal'},'variablenames',{'TrialType','Predictive','Reward'});
- % rm=fitrm(t,'NPSuc-PrMal~subject','WithinDesign',vars);
- % BehavStats.CuedLatency.ranovatbl=ranova(rm);
- % BehavStats.CuedLatency.predictive=multcompare(rm,'Predictive');
- % BehavStats.CuedLatency.reward=multcompare(rm,'Reward');
- % BehavStats.CuedLatency.predictivebyreward=multcompare(rm,'Predictive','by','Reward');
-
- suc_interspersed = median_latencyNo1;
- malt_interspersed = median_latencyNo2;
- suc_blocked = median_latencyPr1;
- malt_blocked = median_latencyPr2;
- [~,BehavStats.CuedLatency.anovatbl,stats] = anovan(cat(1,suc_interspersed,malt_interspersed,suc_blocked,malt_blocked),...
- {cat(1,zeros(num_sessions*2,1),ones(num_sessions*2,1)),...
- cat(1,zeros(num_sessions,1),ones(num_sessions,1),zeros(num_sessions,1),ones(num_sessions,1)),...
- cat(1,rat_name,rat_name,rat_name,rat_name)},...
- 'varnames',{'Non-pr/Pr','Reward','Subject'},'model',[1 0 0;1 1 0;0 0 1],... %[1 0 0;0 1 0;1 1 0;0 0 1],...
- 'random',3,'display','off');
- [BehavStats.CuedLatency.multcompare,BehavStats.CuedLatency.multcompare_means] = multcompare(stats,'dimension',[1],'display','off');
- BehavStats.CuedLatency.stats=stats;
-
- % Trials completed
-
- % t=table(rat_name,fraction_completed(:,1),fraction_completed(:,2),fraction_completed(:,3),fraction_completed(:,4),'variablenames',{'subject','NPSuc','NPMal','PrSuc','PrMal'});
- % vars=table({'NPSuc';'NPMal';'PrSuc';'PrMal'},{'Non'; 'Non'; 'Pr' ;'Pr'},{'Suc'; 'Mal' ;'Suc' ;'Mal'},'variablenames',{'TrialType','Predictive','Reward'});
- % rm=fitrm(t,'NPSuc-PrMal~subject','WithinDesign',vars);
- % BehavStats.CuedCompletion.ranovatbl=ranova(rm);
- % BehavStats.CuedCompletion.predictive=multcompare(rm,'Predictive');
- % BehavStats.CuedCompletion.reward=multcompare(rm,'Reward');
- % BehavStats.CuedCompletion.predictivebyreward=multcompare(rm,'Predictive','by','Reward');
-
-
- suc_inter = fraction_completed(:,1);
- malt_inter = fraction_completed(:,2);
- suc_block = fraction_completed(:,3);
- malt_block = fraction_completed(:,4);
- %
- % figure;
- [~,BehavStats.CuedCompletion.anovatbl,stats] = anovan(cat(1,suc_inter,malt_inter,suc_block,malt_block),...
- {cat(1,zeros(num_sessions*2,1),ones(num_sessions*2,1)),...
- cat(1,zeros(num_sessions,1),ones(num_sessions,1),zeros(num_sessions,1),ones(num_sessions,1)),...
- cat(1,rat_name,rat_name,rat_name,rat_name)},...
- 'varnames',{'Non-pr/Pr','Reward','Subject'},'model',[1 0 0;1 1 0;0 0 1],... %[1 0 0;0 1 0;1 1 0;0 0 1],...
- 'random',3,'display','off');
- [BehavStats.CuedCompletion.multcompare] = multcompare(stats,'dimension',[1 2],'display','off');
- BehavStats.CuedCompletion.stats=stats;
- end
- %lick plots
- %generate lick PSTHs
- for session = 1:num_sessions
-
- % Make logical index vectors to call every event type
- R1 = strcmp('Cue1RD',RAW(session).Einfo(:,2)); %finds the index of the event
- R2 = strcmp('Cue2RD',RAW(session).Einfo(:,2)); %finds the index of the event
- R31 = strcmp('Cue3RD1',RAW(session).Einfo(:,2)); %finds the index of the event
- R32 = strcmp('Cue3RD2',RAW(session).Einfo(:,2)); %finds the index of the event
- Licks = strcmp('Licks',RAW(session).Einfo(:,2)); %finds the index of the event
-
- EvList{1}=RAW(session).Erast{R1};
- EvList{2}=RAW(session).Erast{R2};
- EvList{3}=RAW(session).Erast{R31};
- EvList{4}=RAW(session).Erast{R32};
-
- for k=1:4
- [PSR1,N1]=MakePSR04(RAW(session).Erast(Licks),EvList{k},Dura,{1});% makes collpased rasters. PSR1 is a cell(neurons)
- [PTH1,~,~]=MakePTH07(PSR1,repmat(N1,size(RAW(session).Erast(Licks),1),1),{2,0,BSIZE});%-----Fixed bin used here
- PTH1smooth=[];
- for l=1:length(Tm)
- PTH1smooth(1,l)=sum(PTH1(1,l-min([l-1 smoothbins]):l).*fliplr(filterweights(1:min([l smoothbins+1]))))/sum(filterweights(1:min([l smoothbins+1])));
- end
- Lick.Cued(k).PSTHraw(session,:)=PTH1smooth;
- end
- end
- %plotting
- for type=1:2
-
- psth1=nanmean(Lick.Cued(1+(type-1)*2).PSTHraw(:,Ishow),1);
- sem1=nanste(Lick.Cued(1+(type-1)*2).PSTHraw(:,Ishow),1); %calculate standard error of the mean
- up1=psth1+sem1;
- down1=psth1-sem1;
- psthE=nanmean(Lick.Cued(2+(type-1)*2).PSTHraw(:,Ishow),1);
- semE=nanste(Lick.Cued(2+(type-1)*2).PSTHraw(:,Ishow),1); %calculate standard error of the mean
- upE=psthE+semE;
- downE=psthE-semE;
- %plotting
- subplot(4,4,14+type);
- hold on;
- plot(time1,psth1,'Color',color{1+(type-1)*2},'linewidth',1);
- plot(time1,psthE,'Color',color{2+(type-1)*2},'linewidth',1);
- patch([time1,time1(end:-1:1)],[up1,down1(end:-1:1)],color{1+(type-1)*2},'EdgeColor','none');alpha(0.5);
- patch([time1,time1(end:-1:1)],[upE,downE(end:-1:1)],color{2+(type-1)*2},'EdgeColor','none');alpha(0.5);
- plot([0 0],[-2 8],':','color','k','linewidth',0.75);
- axis([Xaxis(1) Xaxis(2) 0 8]);
- xlabel('Seconds from reward delivery');
- if type==1 title('Cued, predictive'); end
- if type==2 title('Cued, non-predictive'); end
- %legend('Suc trials','Mal trials');
- end
- %% preferences
- %data
- x=[1 2];
- CPref{1}=[83.8 92.7]; %C3
- CPref{2}=[68.7 75.7]; %C4
- CPref{3}=[66.7 90.1]; %C9
- CPref{4}=[96.0 70.7]; %C10
- %CPPref{5}=[82.7 86.9]; %C2
- %CPPref{6}=[77.4 90.2]; %C7
- %not included in ephys
- if strcmp(plot_region,'Both')
- IBPref{1}=[81.7 81.3];
- IBPref{2}=[77 73.9];
- IBPref{3}=[37.4 74.6];
- IBPref{4}=[71 95.6];
- IBPref{5}=[89 83.6];
- IBPref{6}=[94 93];
- IBPref{7}=[59.6 78.8];
- IBPref{8}=[88.8 85.8];
- IBPref{9}=[71.2 89];
- IBPref{10}=[87 76.6];
- IBPref{11}=[85.2 84.1];
- elseif strcmp(plot_region,'VP')
- IBPref{1}=[59.6 78.8];
- IBPref{2}=[88.8 85.8];
- IBPref{3}=[71.2 89];
- IBPref{4}=[87 76.6];
- IBPref{5}=[85.2 84.1];
- elseif strcmp(plot_region,'NA')
- IBPref{1}=[81.7 81.3];
- IBPref{2}=[77 73.9];
- IBPref{3}=[37.4 74.6];
- IBPref{4}=[71 95.6];
- IBPref{5}=[89 83.6];
- IBPref{6}=[94 93];
- end
- subplot(3,5,5);
- hold on;
- %do the first 2 first in order to get the legend right
- plot(x,IBPref{1},'Marker','o','MarkerFaceColor','w','LineWidth',1,'Color','k');
- plot(x,CPref{1},'Marker','o','MarkerFaceColor','k','LineWidth',1,'Color','k');
- for i=2:length(IBPref)
- plot(x,IBPref{i},'Marker','o','MarkerFaceColor','w','LineWidth',1,'Color','k');
- end
- for i=2:length(CPref)
- plot(x,CPref{i},'Marker','o','MarkerFaceColor','k','LineWidth',1,'Color','k');
- end
- axis([0.7 2.2 0 100]);
- title('Two-bottle choice');
- xlabel('Initial Final');
- ylabel('% sucrose consumption');
- plot([0 3],[50 50],':','color','k','linewidth',0.75);
- set(gca,'xtick',[])
- legend('Int / block','Cued','location','southeast');
- end
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