confocal Z-stacks and analysis files of murine brain slices from different brain regions, fluorescently labeled by transgenic markers (glutamaergic, gabaergic and serotonergic neurons) or immunomarkers (NeuN, TH for dopaminergic Neurons, Iba-1 for microglia).

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Satellite_microglia

Imaging files and analysis files relevant for publication Bakina et al "Microglia form satellites with different neuronal subtypes in the adult murine CNS" Murine brain slices from different brain regions were fluorescently labeled by transgenic markers (glutamaergic, gabaergic and serotonergic neurons) or immunomarkers (NeuN, TH for dopaminergic Neurons, Iba-1 for microglia). Confocal Z-stacks were acquired on a Zeiss LSM 700 and 3 D volumes analysed for microglia-neuron soma contacts by IMARIS (surface-to-surface tool) or ImageJ in teh 3 D volumes.

datacite.yml
Title	Satellite Microglia in the mouse CNS # Additional information about the resource, e.g., a brie\| Microglia are the immune cells of the central nervous system. A subpopulation, satellite microglia, form direct contact of their cell body to a neuron cell body. This particular neuron-glia interaction may have implications for neuron function. To understand what factors determine satellite microglia formation we performed a morphological analysis of adult mouse brains and retinae. Satellite microglia occur in all areas containing neuron somata, and their frequency is determined by the soma density of neurons in a given brain region whereas a preference to form a satellite with neurons of a specific neurotransmitter subtype was not observed.
Authors	Nolte,Christiane;Cellular Neurosciences, Max Delbrueck Center for Molecular medicine, Berlin, Germany;ORCID:0000-0001-5398-3440 Kettenmann,Helmut;Shenzhen Institute of Advanced Technology, Chin.Acad.Sci, Shenzhen, China;ResearcherID:X-1234-5678 Bakina,Olga
License	Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References	Citation1 [doi:10.xxx/zzzz] (IsSupplementTo) [] ()
Funding	Institutional funding
Keywords	Neuroscience microglia neuron-glia interaction satellite microglia GABAergic glutamatergic serotonergic dopaminergic image analysis
Resource Type	Dataset

README.md

Satellite_microglia