README.md 579 B
Satellite_microglia
Imaging files and analysis files relevant for publication Bakina et al "Microglia form satellites with different neuronal subtypes in the adult murine CNS" Murine brain slices from different brain regions were fluorescently labeled by transgenic markers (glutamaergic, gabaergic and serotonergic neurons) or immunomarkers (NeuN, TH for dopaminergic Neurons, Iba-1 for microglia). Confocal Z-stacks were acquired on a Zeiss LSM 700 and 3 D volumes analysed for microglia-neuron soma contacts by IMARIS (surface-to-surface tool) or ImageJ in teh 3 D volumes.