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@@ -4,6 +4,12 @@ title: >-
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message: This is our project description
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type: dataset
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authors:
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+ - given-names: Marianna
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+ family-names: Tolve
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+ email: marianna.tolve@uk-koeln.de
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+ orcid: https://orcid.org/0000-0002-0893-2967
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+ affiliation: >-
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+ University of Cologne, Medical Faculty, Center for Physiology & CECAD
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- given-names: Natalia
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family-names: Kononenko
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email: natalia.kononenko@uk-koeln.de
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@@ -14,8 +20,13 @@ authors:
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University Hospital Cologne, University of Cologne, Cologne, Germany
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orcid: https://orcid.org/0000-0002-3425-6659
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abstract: >-
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- This dataset contains live imaging data of activity-dependent intracellular Ca2+
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- dynamics in cerebellar organotypic slices of WT and AP-2KO mice. The slices were
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- transduced with GCAMP7-expressing AAVs and imaged after 3 weeks in vitro with
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- confocal microscopy for 60 seconds. To induce neuronal activity, the slice were
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- electrically stimulated at 100Hz on the 20th second.
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+ The current research proposal focuses on cellular and molecular
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+ mechanisms underlying the maintenance of voluntary motor control by
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+ neurons in cerebellum and basal ganglia in mice and how endocytic
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+ protein AP-2 control this process. This dataset contains live
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+ imaging data of activity-dependent intracellular Ca2+ dynamics in
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+ cerebellar organotypic slices of WT and AP-2KO mice. The slices were
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+ transduced with GCAMP7-expressing AAVs and imaged after 3 weeks in
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+ vitro with confocal microscopy for 60 seconds. To induce neuronal
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+ activity, the slice were electrically stimulated at 100Hz on the
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+ 20th second.
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