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Samhaber_Schottdorf_et_al_2016
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10.12751/g-node.1e7756
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Detailed instructions for building a micro contact printing (μCP) machine and for using it to spatially structure neuronal cultures. Its purpose is to allow any lab without access to a lithography facility to structure cultures of neurons with the method developed in our group. It accompanies the paper R. Samhaber et al. 2016.

You can find a detailed description as to the preparation of neuronal cultures, the protocols used, and the construction of the μCP machine including all CAD files.

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README.md

Samhaber_Schottdorf_et_al_2016

This repository accompanies the manuscript Samhaber et al. J Neurosc Methods 2016 and is a packup for the website with the supplemental online material.

It contains detailed instructions for building a micro contact printing (μCP) machine and for using it to spatially structure neuronal cultures. Its purpose is to allow any lab without access to a lithography facility to structure cultures of neurons with the method developed in our group. You can find a detailed description as to the preparation of neuronal cultures, the protocols used, and the construction of the μCP machine including all CAD files.

The repository contains two archives.

machine.zip

This archive contains the CAD drawings of the stamping mechanism described in the paper. It also contains a detailed part list.

archive_website.tar.gz

This archive contains s a local copy of the supplemental online material.

Additional information

In addition, to the two archives, this repository contains a detailed protocol of the stamping procedure with pictures available as pdf file and a 3D rendering of the CAD files.