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# Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice
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+This repository contains the ephys data related to the article " Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice". We provide 239 optogenetic in vivo recordings of non-anesthetized and urethane-anesthetized mice of control and early stimulated (ES) mice expressing ChR2(ET/TC) in a subset of L2/3 pyramidal neurons in the right hemisphere of the prefrontal cortex achieved by in utero electroporation. Following the structure of the article, the dataset contains recordings of:
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-This repository contains the ephys data related to the article " Transient developmental increase of prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice". We provide 134 optogenetic in vivo recordings of not-anesthetized and urethane-anesthetized mice of three age groups of control and early stimulated (ES) mice expressing ChR2(ET/TC) in a subset of L2/3 pyramidal neurons in the right hemisphere of the prefrontal cortex achieved by in utero electroporation. Following the structure of the article, the dataset contains recordings of:
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+**72 electrophysiological recordings from prefrontal cortex of non-anesthetized mice (Ephys_not-anesthetized):**
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+ - 11x P11-12 control recordings
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+ - 10x P11-12 ES recordings
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+ - 13x P23-26 control recordings
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+ - 14x P23-26 ES recordings
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+ - 12x P38-40 control recordings
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+ - 12x P38-40 ES recordings
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-### **Not-anesthetized**
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+**62 electrophysiological recordings from prefrontal cortex of urethane anesthetized mice (Ephys_not-anesthetized)**
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+ - 10x P11-12 control recordings
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+ - 10x P11-12 ES recordings
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+ - 10x P23-25 control recordings
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+ - 11x P23-25 ES recordings
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+ - 9x P38-40 control recordings
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+ - 12x P38-40 ES recordings
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-* 11 recordings of P11-12 control mice
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-* 10 recordings of P11-12 ES mice
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-* 13 recordings of P23-25 control mice
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-* 14 recordings of P23-25 ES mice
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-* 12 recordings of P38-40 control mice
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-* 12 recordings of P38-40 ES mice
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+**21 electrophysiological recordings from prefrontal cortex with transcranial stimulations (Transcranial_recordings):**
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+ - 13x P7-11 recordings
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+ - 8x P12-16 recordings
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-For all not-anesthetized recordings LFP signals, sorted unit information and timestamps of optogenetic stimulation with 473 nm and 596 nm of 3 ms long 4 Hz pulse and 3s long ramp light stimulation are provided.
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+**8 electrophysiological recordings from prefrontal cortex in PV cre-mice (PV_Photo-tagging):**
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+ - 3x P26 recordings
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+ - 5x P37-40 recordings
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-**LFP:** The group folders contain LFP.dat files with the signal traces of the corresponding recording with a sampling frequency of 1000 Hz recorded in L2/3 of the right hemisphere of the prelimbic subdivision of the prefrontal cortex. Each recording contains a baseline period followed by optogenetic stimulations according following protocol:
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+**25 electrophysiological recordings from prefrontal cortex and Hippocampus of non-anesthetized mice (ES_non-anesthetized_HPstim):**
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+ - 13x P38-40 control recordings
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+ - 12x P38-40 ES recordings
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-1. 30 times, 3 s long, 596 nm stimulations (ramps)
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-2. 30 times, 3 s long, 473 nm stimulations (ramps)
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-3. 360 times, 3 ms long, 473 nm stimulations (square pulses)
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-4. 360 times, 3 ms long, 596 nm stimulations (square pulses)
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+**23 electrophysiological recordings from prefrontal cortex of non-anesthetized mice (oldES_non-anesthetized):**
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+ - 11x P38-40 control recordings
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+ - 12x P38-40 old ES recordings
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-Please consider that stimulation timestamps and detection of unit action potentials have a higher sampling frequency of 32000 Hz
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+**28 electrophysiological recordings from prefrontal cortex during social preference task (ES_social_preference):**
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+ - 15x P38-40 control recordings
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+ - 13x P38-40 ES recordings
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-**SUA:** The group folders also contain SUA.mat files with information about manually sorted single units of the corresponding recording. In sum for all not-anesthetized groups 5716 units were sorted. Spike sorting has been done using klusta (https://klusta.readthedocs.io/en/latest/), and manually curated with Phy (https://phy.readthedocs.io/en/latest/).
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-For every recording, a MATLAB structure is present which provides following information for every sorted unit (Unit_Info):
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-* ClusterID: ID of the unit
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-* Channel: The channel on which the unit has been detected
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-* Timestamps: Timestamps of detected action potential with a sampling frequency of 32000 Hz
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-* Waveform: the average waveform of the unit
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+For detailed information of group generation and recording protocol refer to the article.
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-All not-anesthetized recordings were recorded with a 16 channel Neuronexus 1x16 1-shank electrode with a channel interspace of 100 µm. Channel 1 is the most superficial (closer to brain surface), and 16 the deepest. The position of electrodes has been post-mortem histologycally reconstructed.
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+**LFP:**The group folders contain LFP.dat files with the signal traces of the corresponding recording with a sampling frequency of 1000 Hz recorded in L2/3 of the right hemisphere of the prelimbic subdivision of the prefrontal cortex. Each recording contains a baseline period followed by optogenetic stimulations according following protocol:
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-### **Urethane-anesthetized:**
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+1. 3 s long, 596 nm stimulations (ramps)
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+2. 3 s long, 473 nm stimulations (ramps)
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+3. 3 ms long, 473 nm stimulations (square pulses)
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+4. 3 ms long, 596 nm stimulations (square pulses)
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+5.
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+For each recording a Matlab structure is provided containing the timestamps and kind of stimulation. Please consider that stimulation timestamps and detection of unit action potentials have a higher sampling frequency of 32000 Hz
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-* 10 recordings of P11-12 control mice
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-* 10 recordings of P11-12 ES mice
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-* 10 recordings of P23-25 control mice
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-* 11 recordings of P23-25 ES mice
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-* 9 recordings of P38-40 control mice
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-* 12 recordings of P38-40 ES mice
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+**SUA: **The group folders also contain SUA.mat files with information about manually sorted single units of the corresponding recording. According to the article single unit information is provided for following datasets: Non-anesthetized ES and control mice of all three age groups, recordings with transcranial stimulations and optogenetic recordings in PV cre-mice. Spike sorting has been done using klusta (https://klusta.readthedocs.io/en/latest/), and manually curated with Phy (https://phy.readthedocs.io/en/latest/). For every recording, a Matlab structure is present which provides following information for every sorted unit (Unit_Info):
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-All anesthetized recordings were recorded with two contralateral inserted Neuronexus 4x4 4-shank electrodes. The position of electrodes has been post-mortem histologycally reconstructed. The group folders contain LFP.dat files with the signal traces of the corresponding recording with a sampling frequency of 1000 Hz recorded in L2/3 and L5/6 of the right and left hemisphere of the prelimbic subdivision of the prefrontal cortex. Each recording contains a baseline period followed by optogenetic stimulations according following protocol:
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+• ClusterID: ID of the unit
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+• Channel: The channel on which the unit has been detected
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+• Timestamps: Timestamps of detected action potential with a sampling frequency of 32000 Hz
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+• Waveform: the average waveform of the unit
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-1. 30 times, 3 s long, 596 nm stimulations (ramps)
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-2. 30 times, 3 s long, 473 nm stimulations (ramps)
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-3. 180 rounds of 3 ms long square pulses in different frequencies for 3s with 473 nm
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-4. 180 rounds of 3 ms long square pulses in different frequencies for 3s with 596 nm
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-
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-Stimulation timestamps are provided in the corresponding MATLAB structure for each recording.
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+All non-anesthetized recordings were recorded with a 16 channel Neuronexus 1x16 1-shank electrode with a channel interspace of 100 µm. Channel 1 is the most superficial (closer to brain surface), and 16 the deepest. The position of electrodes has been post-mortem histologycally reconstructed.
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+All anesthetized recordings were recorded with two contralateral inserted Neuronexus 4x4 4-shank electrodes. The position of electrodes has been post-mortem histologycally reconstructed.
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For every question and/or further data request, please don't hesitate in contacting me.
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