Impaired dynamics of precapillary sphincters and pericytes at first-order capillaries predict reduced neurovascular function in the aging mouse brain
Changsi Cai
213f880eb8
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Impaired dynamics of precapillary sphincters and pericytes at first-order capillaries predict reduced neurovascular function in the aging mouse brain
This folder contains both the raw data and code for data analysis.
All raw data related to the publications are collected in the excel file 'All_Data_v2'
There are four rar code packages for data analysis. (1) AmiraDataAnalysis.rar
Please unzip the file and load 'RawDataAnl_Vol3_Allcount3_NEW.m' into MATLAB.
This code helps analyze the Amira 3D vasculature shown in Figure 4.
(2) DiameterMeasurement.rar
Please unzip the file and load 'VesselDiameter_ByArea_GUI_MAIN.m' into MATLAB.
This code helps analyze vessel diameter change in 4D (xyzt) scanning.
(3) IHCAnalysis.rar
Please unzip the file and load 'GliaTrans_POI_GUI_MAIN.m' into MATLAB.
This code helps analyze the alpha-SMA density, pericyte and endothelial cell density from the immunohistochemical images in Figure 1.
(4) MathModel.rar
Please unzip the file and load 'amirachangsianal.py'into Python.
This code makes math model of existing vasculatures to estimate the blood pressure and flow at each vessel segment.
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| Title | Impaired dynamics of precapillary sphincters and pericytes at first-order capillaries predict reduced neurovascular function in the aging mouse brain |
| Authors |
Cai,Changsi;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark;ORCID 0000-0002-7001-610X
Zambach,Stefan Andreas ;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Grubb,Søren;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Tao,Lechan He,Chen;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Lind,Barbara Lykke ;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Thomsen,Kirsten Joan ;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Zhang,Xiao;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Hald,Bjørn Olav ;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Nielsen,Reena Murmu ;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Kim,Keyeon;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Devor,Anna Lønstrup,Micael ;Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark Lauritzen,Martin Johannes |
| Description | The microvascular inflow tract (MIT), comprising the penetrating arterioles, precapillary sphincters, and first-order capillaries, is the bottleneck for brain blood flow and energy supply. Exactly how aging alters the structure and function of the MIT remains unclear. By in vivo 4-dimensional two-photon imaging, we reveal an age-dependent decrease in vaso-responsivity accompanied by a decrease in vessel density close to the arterioles and loss of vascular mural cell processes, though the number of mural cell somas and their αSMA density were preserved. The age-related reduction in vascular reactivity was mostly pronounced at precapillary sphincters, highlighting their crucial role in capillary blood flow regulation. Mathematical modeling revealed impaired pressure and flow control in aged mice during vasoconstriction. Interventions that preserve dynamics of cerebral blood vessels may ameliorate age-related decreases in blood flow and prevent brain frailty. |
| License | Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/) |
| References |
Citation1 [doi:10.xxx/zzzz] (IsSupplementTo)
Citation2 [arxiv:mmmm.nnnn] (IsSupplementTo) Citation3 [pmid:nnnnnnnn] (IsReferencedBy) |
| Funding |
Lundbeck Foundation (R273-2017-1791 and R345-2020-1440)
Danish Medical Research Council (1133-00016A) Alice Brenaa Foundation, Augustinus Foundation (19-2858) A. P. Møller Foundation (20-L-0243) Novo Nordisk foundation (0064289) Nordea Foundation Grant to the Center for Healthy Aging (02-2017-1749) |
| Keywords |
Neuroscience
neurovascular coupling aging Precapillary sphincters pericytes microcirculation capillaries cerebral blood flow |
| Resource Type |
Dataset |