Update 'README.md'

README.md

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 # NATAGING-L01111B
 
-Impaired dynamics of precapillary sphincters and pericytes at first-order capillaries predict reduced neurovascular function in the aging mouse brain
+**Impaired dynamics of precapillary sphincters and pericytes at first-order capillaries predict reduced neurovascular function in the aging mouse brain**
+
+This folder contains both the raw data and code for data analysis.
+
+All raw data related to the publications are collected in the excel file 'All_Data_v2'
+
+There are four rar code packages for data analysis.
+(1) AmiraDataAnalysis.rar
+    Please unzip the file and load 'RawDataAnl_Vol3_Allcount3_NEW.m' into MATLAB.
+    This code helps analyze the Amira 3D vasculature shown in Figure 4.
+    
+(2) DiameterMeasurement.rar
+    Please unzip the file and load 'VesselDiameter_ByArea_GUI_MAIN.m' into MATLAB.
+    This code helps analyze vessel diameter change in 4D (xyzt) scanning.
+    
+(3) IHCAnalysis.rar
+    Please unzip the file and load 'GliaTrans_POI_GUI_MAIN.m' into MATLAB.
+    This code helps analyze the alpha-SMA density, pericyte and endothelial cell density from the immunohistochemical images in Figure 1.
+    
+(4) MathModel.rar
+    Please unzip the file and load 'amirachangsianal.py'into Python.
+    This code makes math model of existing vasculatures to estimate the blood pressure and flow at each vessel segment.
+