NATAGING-L01111B/README.md

NATAGING-L01111B

Impaired dynamics of precapillary sphincters and pericytes at first-order capillaries predict reduced neurovascular function in the aging mouse brain

This folder contains both the raw data and code for data analysis.

All raw data related to the publications are collected in the excel file 'All_Data_v2'

There are four rar code packages for data analysis. (1) AmiraDataAnalysis.rar

Please unzip the file and load 'RawDataAnl_Vol3_Allcount3_NEW.m' into MATLAB.
This code helps analyze the Amira 3D vasculature shown in Figure 4.

(2) DiameterMeasurement.rar

Please unzip the file and load 'VesselDiameter_ByArea_GUI_MAIN.m' into MATLAB.
This code helps analyze vessel diameter change in 4D (xyzt) scanning.

(3) IHCAnalysis.rar

Please unzip the file and load 'GliaTrans_POI_GUI_MAIN.m' into MATLAB.
This code helps analyze the alpha-SMA density, pericyte and endothelial cell density from the immunohistochemical images in Figure 1.

(4) MathModel.rar

Please unzip the file and load 'amirachangsianal.py'into Python.
This code makes math model of existing vasculatures to estimate the blood pressure and flow at each vessel segment.