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@@ -0,0 +1,65 @@
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+Date: 2021-02-12
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+Experiment: WT DREADD awake DG + CA3
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+Experimenter: Mariela
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+Objective: in awake
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+ 4x16 probe in DG
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+ 32 linear 25 um probe in CA3 at 20 deg angle
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+
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+SURGERIES
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+
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+Mouse : 4520 - 3 dots , DOB 16-12-2020 , 23.1 g
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+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
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+Solutions: ACSF 2018
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+
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+
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+10:35 iso induction + bupre
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+11:15 Injection site = (0,0,0) ==> DG marks (-0.75;0.5), (0.25;-0.5),
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+ CA3 marks at (1.25;-0.25), (0.25,1.25) ==> center (0.65;0.60).
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+11:50 craniotomy DG = some brain dammage, CA3 ok
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+12:00 Recovery
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+
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+
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+RECORDINGS
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+
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+
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+Mouse : 4520 - 3 dots , DOB 16-12-2020 , 23.1 g
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+injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
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+
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+13:30 Removed the silicon, OK
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+13:50 zero S404 in the middle of the DG craniotomy. some dammage in brain surface
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+ zero CCO5 in the middle of the CA3 craniotomy
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+ take impedances
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+ S404 (0.00;-0.00,-1.950)
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+ CCO5 (0.09, -0.28, -2.78)
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+14:30 CA1
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+ 1300-1450 CA1 all shanks (shank 4 is the latest to reach CA 1)
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+ SWR go up above the layer, and down below CA1.
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+ DS go down above the layer and up in the 2/3rd of the layer itself
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+ We stop as soon as we hear spike, because they are most likely from CA3
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+ All deepst channel to be up and all the shallow to be down
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+ Heard spikes
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+ -1950 - shanks 1 and 4
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+ Saw dentate spike reversal : yes
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+15:20 CCO5 (0;0;-2.790)
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+ 1520- 1990 CA1
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+ 2650-2780 CA3 (very difficult to see spikes, the signal is very noisy and I can not get it better)
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+ we stopped at -2.78, the two probes are very close to each other.
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+ later on the signal got better and then bad again.
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+16:00 Recording 1
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+16:30 Inject 200uL CNO, then recording 2
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+17:00 Recording 3
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+17:45 remove probes
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+18:00 ket/xyl 0.35ml IP - sac
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+
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+CC05 had noise during several moments of the recordings
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+
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+Notes for histology:
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+4406
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+CA3: 20 deg, DiI (1*1 marks total)
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+DG: 0 deg, DiI x (1*4 marks total)
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+TdTomato
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+
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+
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+
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+
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