Aggiorna 'datacite.yml'

datacite.yml

@@ -37,7 +37,15 @@ title: "RAW DATA for Selective reduction of Ca2+ entry through the human NMDA re
 
 # Additional information about the resource, e.g., a brief abstract.
 description: |
-  RAW DATA (MetaPhluor files; Clampex files)
+  Excessive Ca2+ influx through N-methyl-D-aspartate type glutamate receptors (NMDAR) is associated with excitotoxicity and neuronal death. Thus, a reduction of NMDA-mediated Ca2+ entry could represent a valid neuroprotective strategy. We developed a new two-fluorophore approach to efficiently assess the Ca2+ permeability of ligand-gated ion channels, including NMDARs, in different conditions. This technique was able to discriminate differential Ca2+ permeation through different receptor-channels, and through the same channel in different conditions. With this method we confirmed that EU1794-4, a negative allosteric modulator of NMDARs, decreased their Ca2+ permeability. Furthermore, we measured for the first time the fractional Ca2+ current (Pf, i.e. the percentage of the total current carried by Ca2+ ions) of human NMDARs in the presence of EU1794-4, exhibiting a 40 % reduction in comparison of control conditions. EU1794-4 was also able to reduce NMDA-mediated Ca2+ entry in human neurons derived from induced pluripotent stem cells. This last effect was stronger in the absence of extracellular Mg2+, but still significant in its presence, supporting the hypothesis to use NMDA-selective allosteric modulators to lower Ca2+ influx in human neurons, to prevent Ca2+-dependent excitotoxicity and consequent neurodegeneration. To use data please refer to each figure legend in the associated paper. Data are organized following the figure order (1 figure, 1 directory), with subdirectory including each distinct experimental condition.
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+Data used as control and used in multiple figures are only reported in the directory related to the first figure in which they are displayed.
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+Files including fluorescence microscopy data (concerning all figures) are derived and must be visualized using the Sofware MetaPhluor (Molecular Devices).
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+Files including electrophysiological data (patch-clamp; Fig.5 only) can be visualized by using ClampFit (Molecular Devices).
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+For any further information please contact Prof. Sergio Fucile (sergio.fucile@uniroma1.it)
   
 
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