This data repository holds the dynamic clamp recordings and analysis scripts to test whether cooperative ion channels could produce persistent activity in perirhinal cortex neurons.
Cells were recorded intracellularly and the dynamic clamp was used to emulate the presence of multiple clusters of cooperative ion channels in the membrane. Succesive current pulses were used to test for persistent activity and graded persistent activity.
The stimulation protocols were categorized according to the number of pulses and whether the pulses were de- or hyperpolarizing. Then the firing rate between pulses was estimated to test whether the cells were persistenly active and whether the persistent firing increased (respectively decreased) with the number of applied pulses.
The repository is structured as follows
recordings: each folder in here corresponds to a recording session from the recording software RELACS
metadata: contains a mapping of cells to the above recording sessions and a manually created blacklist of protocols that are excluded from the analysis
scripts: python scripts to read in the recordings, analyse the firing rates and generate a report
out: contains the automatically generated pdf report with an overview of all cells and plots of each stimulation
Tested in Linux
conda env create -f environment.yml