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-Contents of data files submitted with manuscript
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-ED Young et al ?Resolution of subcomponents of synaptic release from
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- post-synaptic currents in rat hair-cell/auditory-nerve fiber synapses?.
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- Young, E.D. et al. Resolution of subcomponents of synaptic release from
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- post-synaptic currents in rat hair-cell/auditory-nerve fiber synapses.
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- BioR?iv 2020.05.13.070920; (2020). doi.org/10.1101/2020.05.13.070920.
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-
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-The data submitted consist of the following Matlab data files (.mat).
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- SW421_dD_dEN_C.mat - a data file containing the analysis of one
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- example experiment (called experiment T in the ms).
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- Fig234data.mat - a data file containing the data plotted in Figure
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- 2D, 3D, and 4.
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-There is also a program FigsDemo() which is a Matlab .m file. When run,
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-this program plots Figures 1A, 2A, 2D, 3A, 3D, 4A, 4B, 4C, and 5A, drawing
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-the data from the two datafiles. The statistical tests described in the ms
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-are repeated here.
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-
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-CONTENTS OF SW421_dD_dEN_C.mat
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-This file contains two structures, dD and dEN. Unidentified entries below
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-are either unused parameters or parameters of the program used to construct
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-the lasso fits.
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-
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->> dD
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-dD = struct with fields:
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-
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- DataID: 'SW421m, start 400 s, 2^25/5 samples' Data identifier
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- ut_s: 1.0000e-04 Time resolution of the data, s
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- filters: [0 0]
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- origdata: [1×1 struct]
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- EvThrK: -30 Theshold, pA, used to ID EPSCs
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- EvSpac_sK: 0.0100
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- DataDC: [6710887×1 double] RAW DATA WAVEFORM, with baseline
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- set to 0
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- EditsZero: []
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- timK: [201×1 double] Time axis for the kernel
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- AvgKernel: [201×1 double] Kernel, computed as the average
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- of monophasic EPSCs
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- SDKernel: [201×1 double] SD of the kernel
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- nevk: 6096 Number of events in the average
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- N0: 2.3597 Estimate of the mean-square
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- baseline (mean(var(ZeroWaves))
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- ZeroWaves: [96×6096 double] 10 ms samples of baseline preceding the EPSCs used in the kernel
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- ZWaveInds: [2×6096 double] Index of the start of each sample (time = index*ut_s)
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-
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->> dEN
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-dEN = struct with fields:
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-
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- DataID: 'SW421m, start 400 s, 2^25/5 samples' Data identifier
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- ut_s: 1.0000e-04 Time resolution of all data, s
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- filters: [0 0]
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- EvThrK: -30
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- EvSpac_sK: 0.0100
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- ClThr: -20 Theshold, pA, used to identify
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- EPSCs after baseline zeroing
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- ClusterParams: [5.0000e-04 0.0200 0.0100]
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- iClust: [11849×3 double] Times of EPSCs, one row/EPSC,
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- contains [time-of-peak time-of-start
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- time of end] in indices = time/ut_s
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- cluspks: [11849×1 double] Peak value (pA) of each EPSC
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- clusArea: [11849×1 double] Area of each EPSC. Multiply by
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- ut_s to obtain charge (pC)
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- deletedCl: [4×2 double]
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- addedCl: [85 78 101]
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- LassoParams: [20 5 0.0300 1 0.0300]
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- xsigL: [6710887×1 double] Deconvolved waveform (green
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- signals in Figs 1, 5)
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- dataOIL: [6710887×1 double] Fit waveform = xsigL convolved
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- with kernel
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- RMSEL: [11849×1 double] Relative MS error of each EPSC
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- RootDCEnergy: [11849×1 double] DC baseline offset energy as a
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- fraction of fit energy
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- nsigpks: [11849×1 double] Number of events in each EPSC
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- yOffL: [11849×1 double] DC baseline offset (pA) of the fit
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- to each EPSC (should be small)
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- iEvClustL: {11849×1 cell} Cell array, one cell for each EPSC, contains timimg information
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- for events in same format as iClust
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- PeakAreas: {11849×1 cell} Area (pA) of each event
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- iClRemoved: {5×1 cell}
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- OverlapElim: {55×1 cell}
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-
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-
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-
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-CONTENTS Fig234data.mat, containing the structure Figure234data
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-
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->> Figure234data
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-Figure234data = struct with fields:
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-
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- ReadMe: 'Data for Figs. 2CD, 3D, 4.' File identifier
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- colmns: [1 2 3 4 5 6 7 8 9 10 11 12] X-axis for Figs 2C, 2D, 3D
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- rowname: {1×8 cell} Brief identifiers for the expts
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- Nevents: [8×12 double] Data for Figs. 2C, 2D, 3D.
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- Mampl: [8×12 double] Event count/EPSC varies down the
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- Marea: [8×12 double] rows, columns are y-axis (number
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- of events, EPSC amplitude,
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- EPSC area).
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- intNN: [583×2 double] Data for Fig 4B. Column 1 is
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- interval to preceding EPSC,
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- column 2 is number of events
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- in this EPSC.
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-intNNsmooth: 33 (not used)
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- cmNsig: [10×8 double] Data for Fig 4A. Event count
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- varies down the rows, one column
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- for each experiment. Data are
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- the mean number of of events.
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- normcm: [1.9756 1.3290 1.3256 1.7276 2.6006 2.4915 1.9579 2.3707]
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- Normalizing factors, means of
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- the columns of cmNsig
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- intArea: [2437×2 double] Data for Fig 4C. same format as
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- intNN, except data are EPSC area
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- intAmpl: [2437×2 double] As for intArea, except data are
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- EPSC peak amplitude. No plot,
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- only statistics
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