|
|
@@ -0,0 +1,77 @@
|
|
|
+# Metadata for DOI registration according to DataCite Metadata Schema 4.1.
|
|
|
+# For detailed schema description see https://doi.org/10.5438/0014
|
|
|
+
|
|
|
+## Required fields
|
|
|
+
|
|
|
+# The main researchers involved. Include digital identifier (e.g., ORCID)
|
|
|
+# if possible, including the prefix to indicate its type.
|
|
|
+authors:
|
|
|
+ -
|
|
|
+ firstname: "Gérard"
|
|
|
+ lastname: "Leboulle"
|
|
|
+ affiliation: "Freie Universität Berlin, Neurobiologie"
|
|
|
+ id: "ORCID:0000-0001-9942-9795"
|
|
|
+ -
|
|
|
+ firstname: "Nora"
|
|
|
+ lastname: "Gehne"
|
|
|
+ affiliation: "Freie Universität Berlin, Neurobiologie"
|
|
|
+
|
|
|
+ -
|
|
|
+ firstname: "Anja"
|
|
|
+ lastname: "Froese"
|
|
|
+ affiliation: "Freie Universität Berlin, Neurobiologie"
|
|
|
+
|
|
|
+ -
|
|
|
+ firstname: "Randolf"
|
|
|
+ lastname: "Menzel"
|
|
|
+ affiliation: "Freie Universität Berlin, Neurobiologie"
|
|
|
+
|
|
|
+
|
|
|
+# A title to describe the published resource.
|
|
|
+title: "In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector."
|
|
|
+
|
|
|
+# Additional information about the resource, e.g., a brief abstract.
|
|
|
+description: |
|
|
|
+ In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells.
|
|
|
+
|
|
|
+# Lit of keywords the resource should be associated with.
|
|
|
+# Give as many keywords as possible, to make the resource findable.
|
|
|
+keywords:
|
|
|
+ - Neuroscience
|
|
|
+ - Insect
|
|
|
+ - Apis mellifera
|
|
|
+ - Honeybee
|
|
|
+ - in-vivo electroporation
|
|
|
+ - viral transduction
|
|
|
+ - eGFP
|
|
|
+
|
|
|
+# License information for this resource. Please provide the license name and/or a link to the license.
|
|
|
+# Please add also a corresponding LICENSE file to the repository.
|
|
|
+license:
|
|
|
+ name: "Creative Commons CC0 1.0 Public Domain Dedication"
|
|
|
+ url: "https://creativecommons.org/publicdomain/zero/1.0/"
|
|
|
+
|
|
|
+## Optional Fields
|
|
|
+
|
|
|
+# Funding information for this resource.
|
|
|
+# Separate funder name and grant number by comma.
|
|
|
+funding:
|
|
|
+ - "DFG, LE1809/1-1"
|
|
|
+ - "DFG, LE1809/1-2"
|
|
|
+
|
|
|
+# Related publications. reftype might be: IsSupplementTo, IsDescribedBy, IsReferencedBy.
|
|
|
+# Please provide digital identifier (e.g., DOI) if possible.
|
|
|
+# Add a prefix to the ID, separated by a colon, to indicate the source.
|
|
|
+# Supported sources are: DOI, arXiv, PMID
|
|
|
+# In the citation field, please provide the full reference, including title, authors, journal etc.
|
|
|
+references:
|
|
|
+ -
|
|
|
+ id: "doi:tba"
|
|
|
+ reftype: "IsSupplementTo"
|
|
|
+ citation: "Citation1"
|
|
|
+
|
|
|
+# Resource type. Default is Dataset, other possible values are Software, DataPaper, Image, Text.
|
|
|
+resourcetype: Dataset
|
|
|
+
|
|
|
+# Do not edit or remove the following line
|
|
|
+templateversion: 1.2
|
Gérard Leboulle