DAndrea2023/datacite.yml

# Metadata for DOI registration according to DataCite Metadata Schema 4.1.
# For detailed schema description see https://doi.org/10.5438/0014

## Required fields

# The main researchers involved. Include digital identifier (e.g., ORCID)
# if possible, including the prefix to indicate its type.
authors:
  -
    firstname: "Tiziano"
    lastname: "D'Andrea"
    affiliation: "Department of Physiology and Pharmacology, Sapienza Rome University, Rome, Italy"
    id: "ORCID:0000-0002-3487-8546"
  -
    firstname: "Maria Cristina"
    lastname: "Benedetti"
    affiliation: "Department of Biology and Biotechnologies “Charles Darwin”, Sapienza Rome University, Rome, Italy"
    id: "ORCID:0000-0002-8022-0323"
  -
    firstname: "Lucia"
    lastname: "Monaco"
    affiliation: "Department of Physiology and Pharmacology, Sapienza Rome University, Rome, Italy"
    id: "ORCID:0000-0002-1986-1711"
  -
    firstname: "Alessandro"
    lastname: "Rosa"
    affiliation: "Department of Biology and Biotechnologies “Charles Darwin”, Sapienza Rome University, Rome, Italy"
    id: "ORCID:0000-0001-9999-7223"
  -
    firstname: "Sergio"
    lastname: "Fucile"
    affiliation: "Department of Physiology and Pharmacology, Sapienza Rome University, Rome, Italy; IRCCS Neuromed, Pozzilli (Is), Italy"
    id: "ORCID:0000-0003-0698-1148"
  
# A title to describe the published resource.
title: "RAW DATA for Selective reduction of Ca2+ entry through the human NMDA receptor - a quantitative study by simultaneous Ca2+ and Na+ imaging"

# Additional information about the resource, e.g., a brief abstract.
description: |
  Excessive Ca2+ influx through N-methyl-D-aspartate type glutamate receptors (NMDAR) is associated
  with excitotoxicity and neuronal death. Thus, a reduction of NMDA-mediated Ca2+ entry could represent
  a valid neuroprotective strategy. We developed a new two-fluorophore approach to efficiently assess
  the Ca2+ permeability of ligand-gated ion channels, including NMDARs, in different conditions.
  This technique was able to discriminate differential Ca2+ permeation through different receptor-channels,
  and through the same channel in different conditions. With this method we confirmed that EU1794-4,
  a negative allosteric modulator of NMDARs, decreased their Ca2+ permeability. Furthermore,
  we measured for the first time the fractional Ca2+ current (Pf, i.e. the percentage of the total current
  carried by Ca2+ ions) of human NMDARs in the presence of EU1794-4, exhibiting a 40 % reduction in comparison
  of control conditions. EU1794-4 was also able to reduce NMDA-mediated Ca2+ entry in human neurons derived
  from induced pluripotent stem cells. This last effect was stronger in the absence of extracellular Mg2+, but
  still significant in its presence, supporting the hypothesis to use NMDA-selective allosteric modulators
  to lower Ca2+ influx in human neurons, to prevent Ca2+-dependent excitotoxicity and consequent neurodegeneration.
  
  To use data please refer to each figure legend in the associated paper. Data are organized following the figure order (1 figure, 1 directory), with subdirectory including each distinct experimental condition.
  Data used as control and used in multiple figures are only reported in the directory related to the first figure in which they are displayed.
  Files including fluorescence microscopy data (concerning all figures) are derived and must be visualized using the Sofware MetaPhluor (Molecular Devices).
  Files including electrophysiological data (patch-clamp; Fig.5 only) can be visualized by using ClampFit (Molecular Devices).
  For any further information please contact Prof. Sergio Fucile (sergio.fucile@uniroma1.it)
  

# Lit of keywords the resource should be associated with.
# Give as many keywords as possible, to make the resource findable.
keywords:
  - Neuroscience
  - Excitotoxicity
  - Neurodegeneration
  - Neuroprotection
  - Ca2+ permeability
  - negative allosteric modulator
  - human iPSCs.

# License information for this resource. Please provide the license name and/or a link to the license.
# Please add also a corresponding LICENSE file to the repository.
license:
  name: "Creative Commons CC0 1.0 Public Domain Dedication"
  url: "https://creativecommons.org/publicdomain/zero/1.0/"



## Optional Fields

# Funding information for this resource.
# Separate funder name and grant number by comma.
funding:
  - "Italian Ministry of Health (Ricerca Corrente)"
 


# Related publications. reftype might be: IsSupplementTo, IsDescribedBy, IsReferencedBy.
# Please provide digital identifier (e.g., DOI) if possible.
# Add a prefix to the ID, separated by a colon, to indicate the source.
# Supported sources are: DOI, arXiv, PMID
# In the citation field, please provide the full reference, including title, authors, journal etc.
references:
-
  id: "doi:tba"
  reftype: "IsSupplementTo"
  citation: "Tiziano DAndrea, Maria Cristina Benedetti, Lucia Monaco, Alessandro Rosa, Sergio Fucile: Selective reduction of Ca2+ entry through the human NMDA receptor: a quantitative study by simultaneous Ca2+ and Na+ imaging. Submitted."

# Resource type. Default is Dataset, other possible values are Software, DataPaper, Image, Text.
resourcetype: Dataset

# Do not edit or remove the following line
templateversion: 1.2