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README.md 90b7783d35 gin commit from rMBP-15-Malthe.local 4 gadi atpakaļ
co_registration.py 90b7783d35 gin commit from rMBP-15-Malthe.local 4 gadi atpakaļ
helper_functions.py 90b7783d35 gin commit from rMBP-15-Malthe.local 4 gadi atpakaļ
main.py 90b7783d35 gin commit from rMBP-15-Malthe.local 4 gadi atpakaļ
slice_to_template.py 90b7783d35 gin commit from rMBP-15-Malthe.local 4 gadi atpakaļ
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README.md

DAPI template pipeline

The original pipeline for pre-processing, reconstructing, and template creation of the DAPI template.

Description

The reconstruction and template creation was run twice. First with the allen reference atlas as reference without non-linear registration steps resulting in an initial DAPI template. The initial DAPI template was used as reference (instead of the Allen reference atlas) in the second run of reconstruction and template creation, resulting in the final DAPI template. The two runs of the pipeline were manually modified.

This pipeline is based on the original data structure of the slice images with a few sanity checks to make sure that all images are present. To run this pipeline with the data structure from the GIN-repository you need to remove these sanity checks, get the mouse ids from a different directory (like tif/), and get all the image paths in each individual tif folder.

Overview

The main script calls the pre_processing, co_registration, and template_creation with appropriate parameters and paths. In pre_processing the original tif image is down-sampled to 8.6x8.6 um and masked according to the biggest (except background) connected component after smoothing and threshold to remove artefact outside of the brain slice. After pre-processing the slices for each brain is reconstructed into 3-dimensional brain volumes. This reconstruction is carried out by iterative registrations (both between volumes and between slice-pairs) to a reference (Allen or DAPI from first iteration). Finally, each reconstructed brain volume is used in the creation of a population-based average in template_creation.

Packages

This pipeline uses the following non-standard packages (package name: version):

nibabel: 2.1.0
nipype: 0.13.1
numpy: 1.15.1
PIL: 5.1.0
scipy: 1.1.0
skimage: 0.14.0
sklearn: 0.19.2

Registration software

A precompiled binary of Advanced Normalization Tools (ANTs) V.2.1.0 for linux/mac was used.

datacite.yml
Title A three-dimensional, population-based average of the C57BL/6 mouse brain from DAPI-stained coronal slices
Authors Stæger,Frederik Filip;Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark;ORCID:0000-0002-2295-8637
Mortensen,Kristian;Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark;ORCID:0000-0003-3726-0781
Kaufmann,Louis;Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
Hirase,Hajime;Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark;ORCID:0000-0003-3806-6905
Sigurdsson,Björn;Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark;ORCID:0000-0002-7484-7779
Nedergaard,Maiken;Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark and Center for Translational Neuromedicine, University of Rochester Medical Center, Rochester, NY 14642, USA.;ORCID:0000-0001-6502-6031
Description The full data set and pipeline for constructing the three-dimensional, population-based average of the C57BL/6 mouse brain form DAPI-stained coronal slices. This repository also contains a python implementation of automatic coronal brain slice segmentation. The data set constitutes of all the raw slice images (.tif) in full resolution, the pre-processed version (.nii), the individually reconstructed brain volumes, and the final population-based average.
License Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References A three-dimensional, population-based average of the C57BL/6 mouse brain from DAPI-stained coronal slices [] (IsDescribedBy)
Funding
Keywords Neuroscience
Mouse brain template
C57BL/6 brain template
DAPI
Population-based average
Automatic segementation
Resource Type Dataset