icephys_mock_repo

Inhibitory plasticity experiment.

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Recorded neurons:

• CA1 pyramidal neurons in voltage clamp held at -50mV.

2 stimulation pathways:

• 1 control pathway: electrical stimulator placed in the pyramidal layer roughgly 100 um from recorded cell to stimulate proximal (basket cell like) inhibition
• 1 light pathway: light stimulation to target optogenetic Channel rhodopsin in PV interneurons. delivered via 470nm light pulse through the objective.

Experiment details:

• Light and control pathways were stimulated in an interleaved fashion with a 5 second interval.
• Each stimulation pathway consisted of 2 stimulations at 50ms interval: 2 action potentials or 2 light pulses.
• After stable baselines in both the control pathway and the light pathway, plasticity protocol was induced.
• After plasticty protocol induced, light and control pathway stimulation resumed as before.
• A 200ms 2mV voltage step is present at 500ms into the recording to monitor series resistance for the experiment.
  

Plasticity protocol:

• The cell was switched from voltage to current clamp.
• 1 light pulse of 5 ms duration was paired with 4 action potentials (action potential interval = 10ms).
• Light was paired with 0ms latency to the first action potential.
• Action potentials induced by current injection into the cell.
• 1 light and 4 action potentials were repeated 100 times with 200ms interval
• The cell was then switched back to voltage clamp and control and light pathway stimulation was resumed.

Folder structure:

• Each folder follows date -> brain slice number -> cell number
• Each experiment has:
  • A labbook: containing basic metadata about the experiment and comments on the experiments.
  • Image of the recorded slice.
  • Raw CSF file containing the raw trace data exported from the aquisition CED Signal software.
  • Raw MAT file containing exported version of the CSF file readable in Matlab.
  • N.b. MAT file is a truncated version of the CSF file to only contain the relevent traces for the experiment.