CA1 pyramidal neurons in voltage clamp held at -50mV.
2 stimulation pathways:
1 control pathway: electrical stimulator placed in the pyramidal layer roughgly 100 um from recorded cell to stimulate proximal (basket cell like) inhibition
1 light pathway: light stimulation to target optogenetic Channel rhodopsin in PV interneurons. delivered via 470nm light pulse through the objective.
Experiment details:
Light and control pathways were stimulated in an interleaved fashion with a 5 second interval.
Each stimulation pathway consisted of 2 stimulations at 50ms interval: 2 action potentials or 2 light pulses.
After stable baselines in both the control pathway and the light pathway, plasticity protocol was induced.
After plasticty protocol induced, light and control pathway stimulation resumed as before.
A 200ms 2mV voltage step is present at 500ms into the recording to monitor series resistance for the experiment.
Plasticity protocol:
The cell was switched from voltage to current clamp.
1 light pulse of 5 ms duration was paired with 4 action potentials (action potential interval = 10ms).
Light was paired with 0ms latency to the first action potential.
Action potentials induced by current injection into the cell.
1 light and 4 action potentials were repeated 100 times with 200ms interval
The cell was then switched back to voltage clamp and control and light pathway stimulation was resumed.
Folder structure:
Each folder follows date -> brain slice number -> cell number
Each experiment has:
A labbook: containing basic metadata about the experiment and comments on the experiments.
Image of the recorded slice.
Raw CSF file containing the raw trace data exported from the aquisition CED Signal software.
Raw MAT file containing exported version of the CSF file readable in Matlab.
N.b. MAT file is a truncated version of the CSF file to only contain the relevent traces for the experiment.
