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+#!/usr/bin/env python3
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+# -*- coding: utf-8 -*-
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+"""
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+Log2List for setupD
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+
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+created June2022, based on pervious VTK2021 log2list
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+@author: galizia
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+
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+Plan:
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+ 1) read a .log file (Till Photonics), create a .lst file for this animal
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+ 2) read a .txt file that comes from PAL/Chronos (Barcode Reader), add odor information to .lst file
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+
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+Structure:
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+ Data should be in subfolder 01_DATA
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+ List file goes into subfolder 02_LISTS
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+
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+Naming:
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+ Within Till, names are ODOR-CONC_NUMBER,
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+ e.g. ISOE-5_10
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+
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+"""
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+
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+
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+from view.python_core.measurement_list import MeasurementList
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+from view.python_core.measurement_list.importers import get_importer_class
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+from view.python_core.flags import FlagsManager
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+from collections import OrderedDict
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+import pandas as pd
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+import logging
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+
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+logging.basicConfig(level=logging.INFO)
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+
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+# ------------------- Some parameters about experimental setup, data structure and output file type --------------------
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+# 3 for single wavelength Till Photonics Measurements
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+# 4 for two wavelength Till Photonics Measurements
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+# 20 for Zeiss Confocal Measurements
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+LE_loadExp = 3
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+
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+# Mother of all Folders of your dataset
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+# On Windows, if you copy paths from the file explorer, make sure the string below is always of the form r"......"
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+#STG_STG_STG_MotherOfAllFolders = r"/home/ajay/Nextcloud/VTK_2021/bee/HS_210521_test"
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+#STG_STG_STG_MotherOfAllFolders = r"/Users/galizia/Documents/DATA/VTK_test/YT_VTK"
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+#STG_STG_STG_MotherOfAllFolders = r"/Users/galizia/Documents/DATA/HS_210521_test"
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+STG_MotherOfAllFolders = r"/Users/galizia/Nextcloud/VTK_2021/Bee_alarm_2022" # 01_DATA
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+
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+
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+# path of the "Data" folder in VIEW organization containing the data
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+# On Windows, if you copy paths from the file explorer, make sure the string below is always of the form r"......"
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+STG_Datapath = r"01_DATA"
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+
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+# path of the "Lists" folder in VIEW organization containing the list files
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+# On Windows, if you copy paths from the file explorer, make sure the string below is always of the form r"......"
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+STG_OdorInfoPath = r"02_LISTS"
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+
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+# Choose measurement list output extension among ".lst", ".lst.xlsx", ".settings.xlsx"
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+# VIEW does not support writing .xls list files anymore (nonetheless, it can read them and revise/update them to .xlsx)
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+measurement_output_extension = ".lst.xlsx"
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+
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+# ------------------- A dictionary containing default values for metadata.----------------------------------------------
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+# ------------------- Only metadata included in this dictionary will be written ----------------------------------------
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+# ----Note that columns of the output measeurement list files will have the same order as below.------------------------
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+
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+default_values = OrderedDict()
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+
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+default_values['Measu'] = 0 # unique identifier for each line, corresponds to item in TILL photonics log file
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+
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+default_values['Label'] = "none"
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+default_values['Odour'] = 'odor?' # stimulus name, maybe extracted from label in the function "custom_func" below
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+default_values['OConc'] = 0 # odor concentration, maybe extracted from label in the function "custom_func" below
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+default_values['Analyze'] = -1 # whether to analyze in VIEWoff. Default 1
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+
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+default_values['Cycle'] = 0 # how many ms per frame
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+default_values['DBB1'] = 'none' # file name of raw data
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+default_values['UTC'] = 0 # recording time, extracted from file
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+
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+default_values['PxSzX'] = '4.6' # um per pixel, 1.5625 for 50x air objective, measured by Hanna Schnell July 2017 on Till vision system, with a binning of 8
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+default_values['PxSzY'] = '4.6' # um per pixel, 1.5625 for 50x air objective, measured by Hanna Schnell July 2017 on Till vision system, with a binning of 8
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+
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+default_values['Lambda'] = 0 # wavelength of stimulus. In TILL, from .log file, In Zeiss LSM, from .lsm file
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+
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+# These will be automatically filed for LE_loadExp=4
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+default_values['dbb2'] = 'none' # file name of raw data in dual wavelength recordings (FURA)
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+# To include more columns, uncomment entries below and specify a default value.
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+# #
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+# block for first stimulus
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+# default_values['StimON'] = -1 # stimulus onset, unit: frames, count starts at frame 1.
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+# default_values['StimOFF'] = -1 # stimulus offset, unit: frames, count starts at frame 1.
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+# default_values['StimLen'] = 0 # stimulus onset in ms from beginning - alternative to StimON
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+# default_values['StimONms'] = -1 # stimulus length in ms - alternative to StimOFF
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+# #
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+# block for second stimulus
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+# default_values['Stim2ON'] = 0 # stimulus onset, unit: frames, count starts at frame 1.
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+# default_values['Stim2OFF'] = 0 # stimulus offset, unit: frames, count starts at frame 1.
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+# default_values['Stim2Len'] = 0 # stimulus onset in ms from beginning - alternative to StimON
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+# default_values['Stim2ONms'] = -1 # stimulus length in ms - alternative to StimOFF
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+# #
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+# default_values['Age'] = -1
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+# default_values['Sex'] = 'o'
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+# default_values['Side'] = 'none'
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+# default_values['Comment'] = 'none'
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+# #
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+# default_values['MTime'] = 0
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+# default_values['Control'] = 0
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+# default_values['Pharma'] = 'none'
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+# default_values['PhTime'] = 0
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+# default_values['PhConc'] = 0
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+# default_values['ShiftX'] = 0
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+# default_values['ShiftY'] = 0
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+# default_values['StimISI'] = 0
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+# default_values['setting'] = 'none'
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+# default_values['dbb3'] = 'none'
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+# default_values['PosZ'] = 0
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+# default_values['Countl'] = 0
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+# default_values['slvFlip'] = 0
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+# ----------------------------------------------------------------------------------------------------------------------
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+
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+# ----------------- A function used to modify list entries after automatic parsing of metadata -------------------------
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+# ----------------- This function indicates what needs to be done for a row --------------------------------------------
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+# ----------------- The same is internally applied to all rows of the measurement list----------------------------------
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+
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+def get_odorinfo_from_label(label):
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+ # format for label is: concentration to the right, with a minus sign
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+ # Odor next to it, separated by underscore
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+ # IMPERARIVE: only ONE "-"
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+
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+ # format ISOE-2_13 or MOL_18
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+
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+ # is the information for a concentration present? Detect "-"
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+ parts = label.split("_")
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+# take first part
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+ parts = parts[0]
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+ parts = parts.split("-")
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+ odor = parts[0]
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+ if len(parts) > 1:
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+ concentration = '-' + parts[1]
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+ else:
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+ concentration = '0'
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+ return [odor, concentration]
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+
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+def custom_func(list_row: pd.Series, animal_tag: str) -> pd.Series:
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+
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+ list_row['StimON'] = 24
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+ list_row['StimLen'] = 1000
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+ list_row['Stim2ON'] = 36
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+ list_row['Stim2Len'] = 1000
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+ list_row['Comment'] = 'AlarmPheromone'
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+ list_row['Line'] = 'bee'
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+ # Examples:
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+ # list_row["StimON"] = 25
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+ (list_row["Odour"],list_row["OConc"]) = get_odorinfo_from_label(list_row["Label"])
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+ # if list_row["Measu"]
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+ # get Odor from another file based on the value of <animal_tag> and list_row["Label"]
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+ return list_row
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+
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+
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+# ----------------------------------------------------------------------------------------------------------------------
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+
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+# ------------------ A function defining the criteria for excluding measurements ---------------------------------------
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+# ------------------ Currently applicable only for tillvision setups ---------------------------------------------------
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+
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+
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+def measurement_filter(s):
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+ # exclude blocks that have in the name "Snapshot" or "Delta"
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+ # or that do not have any "_"
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+ name = s["Label"]
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+ label_not_okay = name.count('Snapshot') > 0 or name.count('Delta') > 0 or name.count('_') < 1
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+ label_okay = not label_not_okay
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+
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+ # exclude blocks with less than two frames or no calibration
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+ atleast_two_frames = False
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+ if type(s["Timing_ms"]) is str:
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+ if len(s["Timing_ms"].split(' ')) >= 2 and s["Timing_ms"] != "(No calibration available)":
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+ atleast_two_frames = True
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+
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+ return label_okay and atleast_two_frames
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+
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+
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+# ______________________________________________________________________________________________________________________
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+
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+
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+# ------------------ names of columns that will be overwritten by old values -------------------------------------------
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+# ------ these will only be used if a measurement list file with the same name as current output file exists -----------
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+
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+overwrite_old_values = ["Line", "PxSzX", "PxSzY", "Age", "Sex", "Prefer",
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+ "Comment", "Analyze", "Odour", "OConc"]
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+
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+# ______________________________________________________________________________________________________________________
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+
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+if __name__ == "__main__":
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+
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+ # initialize a FlagsManager object with values specified above
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+ flags = FlagsManager()
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+ flags.update_flags({"STG_MotherOfAllFolders": STG_MotherOfAllFolders,
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+ "STG_OdorInfoPath": STG_OdorInfoPath,
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+ "STG_Datapath": STG_Datapath})
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+
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+ # initialize importer
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+ importer_class = get_importer_class(LE_loadExp)
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+ importer = importer_class(default_values)
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+
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+ # open a dialog for choosing raw data files
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+ # this returns a dictionary where keys are animal tags (STG_ReportTag) and
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+ # values are lists of associated raw data files
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+ animal_tag_raw_data_mapping = importer.ask_for_files(default_dir=flags["STG_Datapath"])
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+ # make sure some files were chosen
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+ assert len(animal_tag_raw_data_mapping) > 0, IOError("No files were chosen!")
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+
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+ for animal_tag, raw_data_files in animal_tag_raw_data_mapping.items():
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+
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+ # automatically parse metadata
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+ metadata_df = importer.import_metadata(raw_data_files=raw_data_files,
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+ measurement_filter=measurement_filter)
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+ # inform user if no usable measurements were found
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+ if metadata_df.shape[0] == 0:
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+ logging.info(f"No usable measurements we found among the files "
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+ f"chosen for the animal {animal_tag}. Not creating a list file")
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+ else:
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+ # create a new Measurement list object from parsed metadata
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+ measurement_list = MeasurementList.create_from_df(LE_loadExp=LE_loadExp,
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+ df=metadata_df)
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+
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+ # apply custom modifications
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+ measurement_list.update_from_custom_func(custom_func=custom_func, animal_tag=animal_tag)
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+
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+ # set anaylze to 0 if raw data files don't exist
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+ flags.update_flags({"STG_ReportTag": animal_tag})
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+ measurement_list.sanitize(flags=flags,
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+ data_file_extensions=importer.movie_data_extensions)
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+
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+ # construct the name of the output file
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+ out_file = f"{flags.get_lst_file_stem()}{measurement_output_extension}"
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+
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+ # write measurement file to list
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+ measurement_list.write_to_list_file(lst_fle=out_file, columns2write=default_values.keys(),
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+ overwrite_old_values=overwrite_old_values)
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+
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+
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+
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Giovanni Galizia