Tracking_data_and_Analysis_codes_in_Ikarashi_2021

This repository contains R scripts and data used for investigating rhythmic behaivor of fruit flies. Flies and experimental condition should be referred to: Masayoshi Ikarashi and Hiromu Tanimoto (2021) "Drosophila acquires seconds-scale rhythmic behavior"(under revision).

Rdata

This folder contains data, like velocity and result of behavioral classification, that can be analyzed by following R scripts.

Script_Wavelet_Analysis.R

This is a R-script for wavelet analysis. Behavioral output can be also visualized as ethogram or histogram.

The wavelet analysis is performed by using the R-package called "Rwave".

You can also visualize input-signal like behavioral time series and spectrogram by the same script.

https://cran.r-project.org/web/packages/Rwave/index.html

Script_Bootstrapped_SEM.R

This is a R-script for calculating bootstrapped standard error of the means.

You can also visualize the result.

Script_Permutation_Test.R

This is a R-script for performing permutation-test.

Description of variables

In matrix data, each row represents each time point (59.94fps) and each column represents each fly. Fisrt stimulus started in 10 times inter-stimulus-interval (i.e. Firts stimulus started at 20 sec in 2 sec inter-stimulus-group).

*_exp: experimental group

*_con: control group

mat_*: matrix of the resulut of behavioral classification. 1: jumping, 2: walking, 3: small movement, 4: resting.

lv_*: matrix of linear velocity. [millimeter/sec]

side_*: matrix of sideways velocity. [millimeter/sec]

ecc_*: matrix of eccentricity of ellipse.

dir_*: matrix of direction of major axis of ellipse against horizontal axis.