PACK_in-vivo/README.md

Long-term in vivo application of a potassium channel-based optogenetic silencer in the healthy and epileptic mouse hippocampus

Here you find the electrophysiological datasets and Python scripts used to analyze the data, which is underlies the conclusions made in our paper about the application of a novel potassium channel- based optogenetic silencer, PACK, in vivo (Kleis et al., submitted to BMC Biology in 2021). The local field potentials (LFPs) were recorded with a Teflon-coated platinum-iridium wire electrode, which were chronically implanted into the hippocampus of healthy or non-epileptic adult male mice. The LFPs were aquired from freely behaving mice, which were inserted to the recording arena for three hours either without illumination (reference recording) or with an optogenetic stimulation during the second hour. We had six experimental groups, which are presented in the file named "LFP_nomenclature_and_datasets.xlsx". Further details about the experimental groups and methods are presented in the published article.

The datasets contain LFPs resampled at 500 Hz in .h5 file format. For the non-epileptic mice (saline groups), data from one the contralateral hippocampal electrode are presented ('HCc'). For epileptic mice (kainate groups), data from ipsilateral ('HCi') and contralateral hippocampal electrodes are presented.

In the three scripts...