anesthesia_bats/Ephys data/SFig5C.m

close all;
clear;
% plot sPSTH for paired data before and after injection 
load('inj_psth.mat')

pair=psths(:,3)==1;
class_=psths(:,5)==0;
aw_=psths(:,2)==0;
% an=psths(:,2)==1;
aw=logical(pair.*aw_.*class_);
units_aw=psths(aw,1);
units_an=units_aw+1;
an=ismember(psths(:,1),units_an);

% protocols
nav_iso=psths(:,4)==1;
com_iso=psths(:,4)==2;
exp_nav=psths(:,4)==3;
unexp_nav=psths(:,4)==4;
exp_com=psths(:,4)==5;
unexp_com=psths(:,4)==6;

% average PSTHs for all units equally and paired (n=6)
av_psth1=mean(psths(logical(nav_iso.*aw),6:106));
av_psth2=mean(psths(logical(com_iso.*aw),6:106));
av_psth3=mean(psths(logical(exp_nav.*aw),6:106));
av_psth4=mean(psths(logical(unexp_nav.*aw),6:106));
av_psth5=mean(psths(logical(exp_com.*aw),6:106));
av_psth6=mean(psths(logical(unexp_com.*aw),6:106));

av_psth1AN=mean(psths(logical(nav_iso.*an),6:106));
av_psth2AN=mean(psths(logical(com_iso.*an),6:106));
av_psth3AN=mean(psths(logical(exp_nav.*an),6:106));
av_psth4AN=mean(psths(logical(unexp_nav.*an),6:106));
av_psth5AN=mean(psths(logical(exp_com.*an),6:106));
av_psth6AN=mean(psths(logical(unexp_com.*an),6:106));

edges=-0.1:0.002:0.1;

% smooth PSTHs
% y=0.005;
% [av_psth1,~]=ksdensity(av_psth1,edges,'Width', y);
% [av_psth2,~]=ksdensity(av_psth2,edges,'Width', y);
% [av_psth3,~]=ksdensity(av_psth3,edges,'Width', y);
% [av_psth4,~]=ksdensity(av_psth4,edges,'Width', y);
% [av_psth5,~]=ksdensity(av_psth5,edges,'Width', y);
% [av_psth6,~]=ksdensity(av_psth6,edges,'Width', y);
% 
% [av_psth1AN,~]=ksdensity(av_psth1AN,edges,'Width', y);
% [av_psth2AN,~]=ksdensity(av_psth2AN,edges,'Width', y);
% [av_psth3AN,~]=ksdensity(av_psth3AN,edges,'Width', y);
% [av_psth4AN,~]=ksdensity(av_psth4AN,edges,'Width', y);
% [av_psth5AN,~]=ksdensity(av_psth5AN,edges,'Width', y);
% [av_psth6AN,xi]=ksdensity(av_psth6AN,edges,'Width', y);

% psth of an individual unit 
unit=12;
u_=psths(:,1)==unit;
ind_nav=psths(logical(u_.*nav_iso),6:106);
ind_com=psths(logical(u_.*com_iso),6:106);

% plot spectrogram of stimuli
load('stims.mat')
Fs=SoundStruct(1).Fs;
nav_stim=SoundStruct(10).sound;
stim_onset=672009;
w=0.010*Fs;
nav_stim=nav_stim(stim_onset-w:stim_onset+w);
com_stim=SoundStruct(2).sound;
stim_onset=672009;
com_stim=com_stim(stim_onset-w:stim_onset+w);
nwin=64;
noverlap=48;
nfft = 1024; 

%% plotting
figure(1); set(gcf,'Position',[300 150 450 100])
subplot(1,3,1)
plot(edges,av_psth1,'b'); hold on 
plot(edges,av_psth2,'r')
subplot(1,3,2)
plot(edges,av_psth3,'Color',[0.6 0.6 0.6]); hold on
plot(edges,av_psth4,'k')
subplot(1,3,3)
plot(edges,av_psth5,'Color',[0.6 0.6 0.6]); hold on 
plot(edges,av_psth6,'k')
linkaxes()
% xlim([0 100])
% ylim([0 24])

figure(2); set(gcf,'Position',[300 150 450 100])
subplot(1,3,1)
plot(edges,av_psth1AN,'b'); hold on 
plot(edges,av_psth2AN,'r')
subplot(1,3,2)
plot(edges,av_psth3AN,'Color',[0.6 0.6 0.6]); hold on
plot(edges,av_psth4AN,'k')
subplot(1,3,3)
plot(edges,av_psth5AN,'Color',[0.6 0.6 0.6]); hold on 
plot(edges,av_psth6AN,'k')
linkaxes()
% xlim([0 100])
% ylim([0 24])

figure(3); set(gcf,'Position',[300 450 350 150])
subplot(221)
spectrogram(nav_stim, nwin, noverlap, nfft,Fs,'yaxis'); 
colorbar off
caxis([-100, -50])
xlabel([])
ylabel('freq. (kHz)','FontSize',8) 
yticks(0:40:80)
xticks(0:5:20)
xticklabels(-10:5:10)

set(gca, 'box', 'off')
set(gca, 'Color','none')
set(gca,'linewidth',1);set(gca,'fontsize',8);

subplot(222)
spectrogram(com_stim, nwin, noverlap, nfft,Fs,'yaxis'); 
colorbar off
caxis([-100, -50])
colormap(flipud(gray)); 
ylabel([])
xlabel([])
yticks(0:40:80)
xticks(0:5:20)
xticklabels(-10:5:10)

set(gca, 'box', 'off')
set(gca, 'Color','none')
set(gca,'linewidth',1);set(gca,'fontsize',8);


subplot(223)
bar(edges*1000,ind_nav,'k'); 
xline(0,'Color','k','LineWidth',1,'LineStyle',':')
ylabel({'# spikes';'in 2ms'}, 'FontSize', 8)
set(gca, 'box', 'off')
set(gca, 'Color','none')
set(gca,'linewidth',1);set(gca,'fontsize',8);

subplot(224)
bar(edges*1000,ind_com,'k'); 
yticks([])
xline(0,'Color','k','LineWidth',1,'LineStyle',':')
ylim([0 0.7])
set(gca, 'box', 'off')
set(gca, 'Color','none')
set(gca,'linewidth',1);set(gca,'fontsize',8);

exportgraphics(gcf,'E:\Users\User\Desktop\delay paper\anesthetized\figures_paper\ind_unit_iso.pdf',...
    'Resolution',300','ContentType','vector','BackgroundColor','none')