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- # -*- coding: utf-8 -*-
- """
- This is an example for creating simple plots from various Neo structures.
- It includes a function that generates toy data.
- """
- from __future__ import division # Use same division in Python 2 and 3
- import numpy as np
- import quantities as pq
- from matplotlib import pyplot as plt
- import neo
- def generate_block(n_segments=3, n_channels=8, n_units=3,
- data_samples=1000, feature_samples=100):
- """
- Generate a block with a single recording channel group and a number of
- segments, recording channels and units with associated analog signals
- and spike trains.
- """
- feature_len = feature_samples / data_samples
- # Create container and grouping objects
- segments = [neo.Segment(index=i) for i in range(n_segments)]
- chx = neo.ChannelIndex(index =1, name='T0')
- for i in range(n_channels):
- rc = neo.RecordingChannel(name='C%d' % i, index=i)
- rc.channelindexes = [chx]
- chx.recordingchannels.append(rc)
- units = [neo.Unit('U%d' % i) for i in range(n_units)]
- chx.units = units
- block = neo.Block()
- block.segments = segments
- block.channel_indexes = [chx]
- # Create synthetic data
- for seg in segments:
- feature_pos = np.random.randint(0, data_samples - feature_samples)
- # Analog signals: Noise with a single sinewave feature
- wave = 3 * np.sin(np.linspace(0, 2 * np.pi, feature_samples))
- for rc in chx.recordingchannels:
- sig = np.random.randn(data_samples)
- sig[feature_pos:feature_pos + feature_samples] += wave
- signal = neo.AnalogSignal(sig * pq.mV, sampling_rate=1 * pq.kHz)
- seg.analogsignals.append(signal)
- rc.analogsignals.append(signal)
- # Spike trains: Random spike times with elevated rate in short period
- feature_time = feature_pos / data_samples
- for u in units:
- random_spikes = np.random.rand(20)
- feature_spikes = np.random.rand(5) * feature_len + feature_time
- spikes = np.hstack([random_spikes, feature_spikes])
- train = neo.SpikeTrain(spikes * pq.s, 1 * pq.s)
- seg.spiketrains.append(train)
- u.spiketrains.append(train)
- block.create_many_to_one_relationship()
- return block
- block = generate_block()
- # In this example, we treat each segment in turn, averaging over the channels
- # in each:
- for seg in block.segments:
- print("Analysing segment %d" % seg.index)
- siglist = seg.analogsignals
- time_points = siglist[0].times
- avg = np.mean(siglist, axis=0) # Average over signals of Segment
- plt.figure()
- plt.plot(time_points, avg)
- plt.title("Peak response in segment %d: %f" % (seg.index, avg.max()))
- # The second alternative is spatial traversal of the data (by channel), with
- # averaging over trials. For example, perhaps you wish to see which physical
- # location produces the strongest response, and each stimulus was the same:
- # We assume that our block has only 1 ChannelIndex and each
- # RecordingChannel only has 1 AnalogSignal.
- chx = block.channel_indexes[0]
- for rc in chx.recordingchannels:
- print("Analysing channel %d: %s" % (rc.index, rc.name))
- siglist = rc.analogsignals
- time_points = siglist[0].times
- avg = np.mean(siglist, axis=0) # Average over signals of RecordingChannel
- plt.figure()
- plt.plot(time_points, avg)
- plt.title("Average response on channel %d" % rc.index)
- # There are three ways to access the spike train data: by Segment,
- # by RecordingChannel or by Unit.
- # By Segment. In this example, each Segment represents data from one trial,
- # and we want a peristimulus time histogram (PSTH) for each trial from all
- # Units combined:
- for seg in block.segments:
- print("Analysing segment %d" % seg.index)
- stlist = [st - st.t_start for st in seg.spiketrains]
- count, bins = np.histogram(np.hstack(stlist))
- plt.figure()
- plt.bar(bins[:-1], count, width=bins[1] - bins[0])
- plt.title("PSTH in segment %d" % seg.index)
- # By Unit. Now we can calculate the PSTH averaged over trials for each Unit:
- for unit in block.list_units:
- stlist = [st - st.t_start for st in unit.spiketrains]
- count, bins = np.histogram(np.hstack(stlist))
- plt.figure()
- plt.bar(bins[:-1], count, width=bins[1] - bins[0])
- plt.title("PSTH of unit %s" % unit.name)
- # By ChannelIndex. Here we calculate a PSTH averaged over trials by
- # channel location, blending all Units:
- for chx in block.channel_indexes:
- stlist = []
- for unit in chx.units:
- stlist.extend([st - st.t_start for st in unit.spiketrains])
- count, bins = np.histogram(np.hstack(stlist))
- plt.figure()
- plt.bar(bins[:-1], count, width=bins[1] - bins[0])
- plt.title("PSTH blend of recording channel group %s" % chx.name)
- plt.show()
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