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- Date: 2021-02-11
- Experiment: WT DREADD awake DG + CA3
- Experimenter: Mariela
- Objective: in awake
- 4x16 probe in DG
- 32 linear 25 um probe in CA3 at 20 deg angle
- SURGERIES
- Mouse : 4496 - 1 dot , DOB 16-12-2020 , 23.4 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- Solutions: ACSF 2018
- 10:15 iso induction + bupre
- 11:05 Injection site = (0,0,0) ==> DG marks (-0.75;0.5), (0.25;-0.5),
- CA3 marks at (1.25;-0.25), (0.25,1.25) ==> center (0.72;0.60).
- 11:50 craniotomy DG = ok, CA3 ok
- 12:15 Recovery
- RECORDINGS
- Mouse : 4496 - 1 dot , DOB 16-12-2020 , 23.4 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- 13:45 Removed the silicon, OK
- 14:05 zero S404 in the middle of the DG craniotomy. some bleeding
- zero CCO5 in the middle of the CA3 craniotomy
- take impedances
- S404 (0.01;-0.08,-2.21)
- 14:50 CA1
- 1310-1550 CA1 all shanks (shank 4 is the latest to reach CA 1)
- SWR go up above the layer, and down below CA1.
- DS go down above the layer and up in the 2/3rd of the layer itself
- We stop as soon as we hear spike, because they are most likely from CA3
- All deepst channel to be up and all the shallow to be down
- Heard spikes
- 2110 - shanks 1, 2 and 3
- Saw dentate spike reversal : yes
- 15:40 CCO5 (0;0;-2.790)
- 1490- 1750 CA1
- 2500-2790 CA3
- we stopped at -2.79
- There is noise, ears or hair touching probe or head bar???? check grounding of the probe???
- 16:30 Recording 1
- 17:00 Inject 200uL CNO, then recording 2
- 17:30 Recording 3
- 18:15 remove probes
- 18:30 ket/xyl 0.35ml IP - sac
- CC05 had noise during several moments of the recordings (hair/ear touching the probe?)
- Notes for histology:
- 4406
- CA3: 20 deg, DiI (1*1 marks total)
- DG: 0 deg, DiI x (1*4 marks total)
- TdTomato
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