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- Date: 2021-02-10
- Experiment: WT DREADD awake DG + CA3
- Experimenter: Mariela
- Objective: in awake
- 4x16 probe in DG
- 32 linear 25 um probe in CA3 at 20 deg angle
- SURGERIES
- Mouse : 4497 - 2 dots , DOB 16-12-2020 , 22.8 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- Solutions: ACSF 2018
- 10:40 iso induction + bupre
- 11:05 Injection site = (0,0,0) ==> DG marks (-0.75;0.5), (0.25;-0.5),
- CA3 marks at (1.25;-0.25), (0.25,1.25) ==> center (0.92;0.63).
- 12:20 craniotomy DG = oedema from the injection scar, CA3 ok
- 12:25 Recovery
- RECORDINGS
- Mouse : 4497 - 2 dots , DOB 16-12-2020 , 22.8 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- 14:00 Removed the silicon, OK
- 14:35 zero S404 in the middle of the DG craniotomy
- zero CCO5 in the middle of the CA3 craniotomy
- take impedances
- S404 (0;0,-2.25)
- 15:15 CA1
- 1370-1500 CA1 all shanks
- SWR go up above the layer, and down below CA1.
- DS go down above the layer and up in the 2/3rd of the layer itself
- CHANGED approached about DG: as previous experiments were too lareral and too deep
- we stop as soon as we hear spike, because they are most likely from CA3
- All deepst channel to be up and all the shallow to be down
- Heard spikes
- 2150 - shank 3 and 4
- Saw dentate spike reversal : yes
- 16:10 CCO5 (0;0;-2.78)
- 1570-1950 CA1
- 2600-2785 CA3
- we stopped at -2.78
- 17:22 Recording 1
- 17:55 Inject 200uL saline, then recording 2
- 18:25 Recording 3
- 19:10 remove probes
- 19:20 Iso - sac
- CC05 had noise during several moments of the recordings (hair touchong the probe?)
- Notes for histology:
- 4406
- CA3: 20 deg, DiI (1*1 marks total)
- DG: 0 deg, DiI x (1*4 marks total)
- TdTomato
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