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- Date: 2021-02-08
- Experiment: WT DREADD awake DG + CA3
- Experimenter: Cath
- Objective: in awake
- 4x16 probe in DG
- 32 linear 25 um probe in CA3 at 20 deg angle
- SURGERIES
- Mouse : 4498 - 3 dot , DOB 16-12-2020 , 23.0 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- Solutions: ACSF 2018
- 11:20 iso induction + bupre
- 11:35 Injection site = (0,0,0) ==> DG marks (0.5;-0.5), (-0.5;0),
- CA3 marks at (1.5;0.25), (0.25,1.5) ==> center (0.85;0.88).
- 12:10 craniotomy DG = oedema from the injection scar, CA3 ok
- 12:15 Recovery
- RECORDINGS
- Mouse : 4498 - 3 dot , DOB 16-12-2020 , 23.0 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- 14:40 Removed the silicon, OK
- 15:15 zero S404 in the middle of the DG craniotomy
- zero CCO5 in the middle of the CA3 craniotomy
- take impedances
- I waited 15 minutes after putting the two probes at -800um
- S404 (0.00;-0.00,-2.07)
- 15:20 CA1
- 1230-1500 CA1 all shanks
- SWR go up above the layer, and down below CA1.
- DS go down above the layer and up in the 2/3rd of the layer itself
- CHANGED approached about DG: as previous experiments were too lareral and too deep
- we stop as soon as we hear spike, because they are most likely from CA3
- All deepst channel to be up and all the shallow to be down
- Heard spikes
- 2075 - shank 1
- Saw dentate spike reversal : no
- Channel A60 (deepest of shank 1) in CA3?
- 15:55 CCO5 (0;0;-2.55)
- 18 CA1
- we stopped at -2.55 as we think that the probe is lateral to CA3.
- 17:15 Recording 1
- 17:45 Inject 200uL saline, then recording 2
- 18:17 Recording 3
- 18:50 remove probes
- 19:00 K/X 0.35ml IP - sac
- Notes for histology:
- 4406
- CA3: 20 deg, DiI (1*1 marks total)
- DG: 0 deg, DiI x (1*4 marks total)
- TdTomato
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