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- Date: 2021-01-15
- Experiment: WT DREADD awake DG + CA3
- Experimenter: Mariela
- Objective: in awake
- 4x16 probe in DG
- 32 linear 50 um probe in CA3 at 20 deg angle
- SURGERIES
- Mouse 1: 4400 - 1 dot , DOB 13-12-2020 , 24.6 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- Solutions: serum phy from Upcare
- 10:30 iso induction + bupre
- 11:30 Injection site = (0,0,0)==> DG marks (0.5;-0.5), (-0.5;0.5),
- CA3 marks at (1.5;0.25), (0.25,1.5) ==> center (0.79;0.78).
- 13:00 craniotomies good
- 13:05 Recovery
- RECORDINGS
- Mouse 1: 4400 - 1 dot , DOB 13-12-2020 , 24.6 g
- injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry
- 14:30 Removed the silicon, OK
- 14:50 zero Q631 in the middle of the DG craniotomy
- zero CCO5 in the middle of the CA3 craniotomy
- take impedances
- Look for DS on probe Q631, ideally up in the depest shanks, and down on the shallow.
- Q631 (0;0;-2.5)
- 15:10 CA1
- Heard layer around 1100-1250 - not CA1 but very strong spikes
- Heard layer around 1350-1640 CA1
- SWR go up above the layer, and down below CA1.
- DS go down above the layer and up in the 2/3rd of the layer itself
- All deepst channel to be up and all the shallow to be down
- We want all up before 200uM
- granule cell layer - spikes on :
- 2340-2450- shank 1
- 2370-2500- shank 2
- 2360-2500- shank 3
- 2390-2500- shank 4
- Reversal seems to happen within the upper part of the DG cellular layer.
- 16:20 CCO5 (0;0;-2.32)
- 1570-1670 CA1
- CA3
- 2190-
- 16:30 Recording 1
- 17:00 Inject 100uL CNO + recording 2
- A47 channel seems broken
- 17:30 Recording 3
- 17:50 remove probes
- 17:55 iso 5% - sac
- Notes for histology:
- 4400
- CA3: 20 deg, DiI (1*1 marks total)
- DG: 0 deg, DiI x (1*4 marks total)
- TdTomato
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