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2021-02-10.txt

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  1. Date: 2021-02-10
    2. 

  2. Experiment: WT DREADD awake DG + CA3
    3. 

  3. Experimenter: Mariela
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  4. Objective: in awake
    5. 

  5. 	4x16 probe in DG
    6. 

  6. 	32 linear 25 um probe in CA3 at 20 deg angle
    7. 

  7. 

  8. SURGERIES
    9. 

  9. 

  10. Mouse : 4497 - 2 dots , DOB 16-12-2020 , 22.8 g
    11. 

  11. injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
    12. 

  12. Solutions: ACSF 2018
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  13. 

  14. 

  15. 10:40	iso induction + bupre 
    16. 

  16. 11:05	Injection site = (0,0,0) ==> DG marks (-0.75;0.5), (0.25;-0.5), 
    17. 

  17. 	CA3 marks at (1.25;-0.25), (0.25,1.25) ==> center (0.92;0.63). 
    18. 

  18. 12:20	craniotomy DG = oedema from the injection scar, CA3 ok 
    19. 

  19. 12:25	Recovery 
    20. 

  20. 

  21. 

  22. RECORDINGS
    23. 

  23. 

  24. 

  25. Mouse : 4497 - 2 dots , DOB 16-12-2020 , 22.8 g
    26. 

  26. injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
    27. 

  27. 

  28. 14:00	Removed the silicon, OK
    29. 

  29. 14:35	zero S404 in the middle of the DG craniotomy 
    30. 

  30. 	zero CCO5 in the middle of the CA3 craniotomy
    31. 

  31. 	take impedances
    32. 

  32. 	S404 (0;0,-2.25)
    33. 

  33. 15:15	CA1
    34. 

  34. 	1370-1500 CA1 all shanks
    35. 

  35. 	SWR go up above the layer, and down below CA1. 
    36. 

  36. 	DS go down above the layer and up in the 2/3rd  of the layer itself
    37. 

  37. 	CHANGED approached about DG: as previous experiments were too lareral and too deep
    38. 

  38. 	we stop as soon as we hear spike, because they are most likely from CA3
    39. 

  39. 	All deepst channel to be up and all the shallow to be down
    40. 

  40. 	Heard spikes 
    41. 

  41. 	2150 - shank 3 and 4
    42. 

  42. 	Saw dentate spike reversal : yes 
    43. 

  43. 16:10 	CCO5 (0;0;-2.78)
    44. 

  44. 	1570-1950 CA1
    45. 

  45. 	2600-2785 CA3
    46. 

  46. 	we stopped at -2.78
    47. 

  47. 17:22	Recording 1
    48. 

  48. 17:55	Inject 200uL saline, then recording 2
    49. 

  49. 18:25 	Recording 3
    50. 

  50. 19:10	remove probes	
    51. 

  51. 19:20	Iso - sac
    52. 

  52. 

  53. CC05 had noise during several moments of the recordings (hair touchong the probe?)
    54. 

  54. 

  55. Notes for histology:
    56. 

  56. 4406
    57. 

  57. CA3: 20 deg, DiI (1*1 marks total)
    58. 

  58. DG: 0 deg, DiI x (1*4 marks total)
    59. 

  59. TdTomato
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  60. 

  61. 

  62. 

  63. 

  64.