2024_Ruthe_SND/code/overlayPlot.py

import nibabel as nib
import numpy as np
import matplotlib.pyplot as plt
from scipy.ndimage import rotate

# Define the file paths
templateFilePath = r"C:\Users\aswen\Desktop\Code\2024_Ruthe_SND\input\average_template_50_from_website.nii.gz"
densityFilePath = r"C:\Users\aswen\Desktop\Code\2024_Ruthe_SND\input\12_wks_coronal_100141780_50um_projection_density.nii.gz"
maskFilePath = r"C:\Users\aswen\Desktop\Code\2024_Ruthe_SND\output\Nifti_Trakte_registered\CC_Mop_desnitymask_50um.nii.gz"

# Load the NIfTI files
templateNifti = nib.load(templateFilePath)
densityNifti = nib.load(densityFilePath)
maskNifti = nib.load(maskFilePath)

# Ensure that both images have the same data type (e.g., cast density to the data type of template)
densityNifti = nib.Nifti1Image(densityNifti.get_fdata().astype(templateNifti.get_fdata().dtype), densityNifti.affine)

# Customize the figure DPI
dpi = 400

# Get the number of slices along each dimension
num_slices_dim0, num_slices_dim1, num_slices_dim2 = templateNifti.shape

# Set the slice step for display (every tenth slice)
slice_step = 10

# Choose the dimension for iteration (0, 1, or 2)
iteration_dimension = 0  # Change this value to select the dimension

# Calculate the number of rows and columns for the grid based on the selected dimension
if iteration_dimension == 0:
    num_slices = num_slices_dim0
elif iteration_dimension == 1:
    num_slices = num_slices_dim1
else:
    num_slices = num_slices_dim2

grid_size = int(np.sqrt(num_slices // slice_step))
if grid_size * grid_size < num_slices // slice_step:
    grid_size += 1

cm = 1/2.54
# Adjust the figure size to be larger
fig, axs = plt.subplots(grid_size, grid_size, figsize=(20, 20), dpi=dpi)

# Hide axis numbers
for ax_row in axs:
    for ax in ax_row:
        ax.axis('off')

# Load the mask and perform necessary flips
mask_data = np.squeeze(maskNifti.get_fdata())
# Swap the mask over the x and y axes
mask_data = mask_data.swapaxes(0, 2)
mask_data = np.flip(mask_data, axis=0)

# Loop through and display every nth slice as subplots along the selected dimension
for i, sliceIdx in enumerate(range(0, num_slices, slice_step)):
    row = i // grid_size
    col = i % grid_size

    # Extract the desired slice from the template and density based on the selected dimension
    if iteration_dimension == 0:
        templateSlice = np.squeeze(templateNifti.get_fdata()[sliceIdx, :, :]).astype(np.uint16)
        densitySlice = np.squeeze(densityNifti.get_fdata()[sliceIdx, :, :]).astype(np.double)
        maskSlice = np.squeeze(mask_data[sliceIdx, :, :])
    elif iteration_dimension == 1:
        templateSlice = np.squeeze(templateNifti.get_fdata()[:, sliceIdx, :]).astype(np.uint16)
        densitySlice = np.squeeze(densityNifti.get_fdata()[:, sliceIdx, :]).astype(np.double)
        maskSlice = np.squeeze(mask_data[:, sliceIdx, :])
    else:
        templateSlice = np.squeeze(templateNifti.get_fdata()[:, :, sliceIdx]).astype(np.uint16)
        densitySlice = np.squeeze(densityNifti.get_fdata()[:, :, sliceIdx]).astype(np.double)
        maskSlice = np.squeeze(mask_data[:, :, sliceIdx])

    # Normalize the templateSlice to [0, 1] for visualization
    templateSlice = (templateSlice - np.min(templateSlice)) / (np.max(templateSlice) - np.min(templateSlice))

    # Overlay the density and mask onto the template slice
    overlayedImage = templateSlice + densitySlice + maskSlice

    # Display the overlaid image in the corresponding subplot
    axs[row, col].imshow(templateSlice, cmap='gray')
    axs[row, col].imshow(densitySlice, cmap='hot', alpha=0.3)
    axs[row, col].imshow(maskSlice, cmap='cool', alpha=0.3)  # Apply "hot" colormap to the mask
  # Apply "cool" colormap to the mask

# Remove empty subplots if necessary
for i in range(num_slices // slice_step, grid_size * grid_size):
    fig.delaxes(axs.flatten()[i])

# Adjust spacing between subplots
plt.tight_layout()

# Show the plot
plt.show()