""" This is an example for creating simple plots from various Neo structures. It includes a function that generates toy data. """ import numpy as np import quantities as pq from matplotlib import pyplot as plt import neo def generate_block(n_segments=3, n_channels=4, n_units=3, data_samples=1000, feature_samples=100): """ Generate a block with a single recording channel group and a number of segments, recording channels and units with associated analog signals and spike trains. """ feature_len = feature_samples / data_samples # Create Block to contain all generated data block = neo.Block() # Create multiple Segments block.segments = [neo.Segment(index=i) for i in range(n_segments)] # Create multiple ChannelIndexes block.channel_indexes = [neo.ChannelIndex(name='C%d' % i, index=i) for i in range(n_channels)] # Attach multiple Units to each ChannelIndex for channel_idx in block.channel_indexes: channel_idx.units = [neo.Unit('U%d' % i) for i in range(n_units)] # Create synthetic data for seg in block.segments: feature_pos = np.random.randint(0, data_samples - feature_samples) # Analog signals: Noise with a single sinewave feature wave = 3 * np.sin(np.linspace(0, 2 * np.pi, feature_samples)) for channel_idx in block.channel_indexes: sig = np.random.randn(data_samples) sig[feature_pos:feature_pos + feature_samples] += wave signal = neo.AnalogSignal(sig * pq.mV, sampling_rate=1 * pq.kHz) seg.analogsignals.append(signal) channel_idx.analogsignals.append(signal) # Spike trains: Random spike times with elevated rate in short period feature_time = feature_pos / data_samples for u in channel_idx.units: random_spikes = np.random.rand(20) feature_spikes = np.random.rand(5) * feature_len + feature_time spikes = np.hstack([random_spikes, feature_spikes]) train = neo.SpikeTrain(spikes * pq.s, 1 * pq.s) seg.spiketrains.append(train) u.spiketrains.append(train) block.create_many_to_one_relationship() return block block = generate_block() # In this example, we treat each segment in turn, averaging over the channels # in each: for seg in block.segments: print("Analysing segment %d" % seg.index) siglist = seg.analogsignals time_points = siglist[0].times avg = np.mean(siglist, axis=0) # Average over signals of Segment plt.figure() plt.plot(time_points, avg) plt.title("Peak response in segment %d: %f" % (seg.index, avg.max())) # The second alternative is spatial traversal of the data (by channel), with # averaging over trials. For example, perhaps you wish to see which physical # location produces the strongest response, and each stimulus was the same: # There are multiple ChannelIndex objects connected to the block, each # corresponding to a a physical electrode for channel_idx in block.channel_indexes: print("Analysing channel %d: %s" % (channel_idx.index, channel_idx.name)) siglist = channel_idx.analogsignals time_points = siglist[0].times avg = np.mean(siglist, axis=0) # Average over signals of RecordingChannel plt.figure() plt.plot(time_points, avg) plt.title("Average response on channel %d" % channel_idx.index) # There are three ways to access the spike train data: by Segment, # by ChannelIndex or by Unit. # By Segment. In this example, each Segment represents data from one trial, # and we want a peristimulus time histogram (PSTH) for each trial from all # Units combined: for seg in block.segments: print("Analysing segment %d" % seg.index) stlist = [st - st.t_start for st in seg.spiketrains] count, bins = np.histogram(np.hstack(stlist)) plt.figure() plt.bar(bins[:-1], count, width=bins[1] - bins[0]) plt.title("PSTH in segment %d" % seg.index) # By Unit. Now we can calculate the PSTH averaged over trials for each Unit: for unit in block.list_units: stlist = [st - st.t_start for st in unit.spiketrains] count, bins = np.histogram(np.hstack(stlist)) plt.figure() plt.bar(bins[:-1], count, width=bins[1] - bins[0]) plt.title("PSTH of unit %s" % unit.name) # By ChannelIndex. Here we calculate a PSTH averaged over trials by # channel location, blending all Units: for chx in block.channel_indexes: stlist = [] for unit in chx.units: stlist.extend([st - st.t_start for st in unit.spiketrains]) count, bins = np.histogram(np.hstack(stlist)) plt.figure() plt.bar(bins[:-1], count, width=bins[1] - bins[0]) plt.title("PSTH blend of recording channel group %s" % chx.name) plt.show()