|
@@ -1,3 +1,124 @@
|
|
|
# GammaDevelopment
|
|
|
|
|
|
-Ephys data development P5-40
|
|
|
+Ephys data development P5-40
|
|
|
+
|
|
|
+Elecrophysiological data used for manuscript
|
|
|
+'Gamma activity accelerates during prefrontal development'
|
|
|
+Sebastian H. Bitzenhofer, Jastyn A. Pöpplau, Ileana L. Hanganu-Opatz
|
|
|
+
|
|
|
+
|
|
|
+
|
|
|
+------------- INFORMATION ABOUT FILES -------------
|
|
|
+
|
|
|
+
|
|
|
+--------------------------------------------------------
|
|
|
+--------------------------------------------------------
|
|
|
+--- ExperimentsOverview.xlsx
|
|
|
+
|
|
|
+Summary of animals, recording configurations, optogenetic stimulations, and analysis
|
|
|
+
|
|
|
+
|
|
|
+--------------------------------------------------------
|
|
|
+--------------------------------------------------------
|
|
|
+--- StimulationPropertiesBaselinePeriods
|
|
|
+
|
|
|
+folder structure containing .mat files for each recording (subfolders named by animalID from ExperimentsOverview.xlsx)
|
|
|
+
|
|
|
+- BaselinePeriods.mat
|
|
|
+cell
|
|
|
+contains information about period where spontaneous activity without stimulation was recorded (about 15 min)
|
|
|
+Information is strucutred as follows (columns) (times and samples refer to start of recording as 0 ms / sample 1)
|
|
|
+{1} BaslineStart (in ms)
|
|
|
+{2} BaselineEnd (in ms)
|
|
|
+{3} BaselinDuration (in minutes)
|
|
|
+{4} BaselinStart (in samples)
|
|
|
+{5} BaselinEnd (in samples)
|
|
|
+{6} BaselinDuration (in samples)
|
|
|
+{7} SamplingRate (in samples per s)
|
|
|
+
|
|
|
+- StimulationProperties.mat
|
|
|
+cell
|
|
|
+contains information about optogenetic stimulations
|
|
|
+Further information (Laser power, wavelength, ...) about type of stimulation can be found in ExperimentsOverview.xlsx
|
|
|
+Each row represents one stimulation
|
|
|
+Information is strucutred as follows (columns) (times and samples refer to start of recording as 0 ms / sample 1)
|
|
|
+{1} StimulationStart (in ms)
|
|
|
+{2} StimulationEnd (in ms)
|
|
|
+{3} StimulationDuration (in s)
|
|
|
+{4} Number of pulses (1 for ramp stimulation)
|
|
|
+{5} Duration per pulse (in ms)
|
|
|
+{6} Pulse frequency (in Hz)
|
|
|
+{7} Laser power (set manually on device for ramp stimulations, see inforamtion in ExperimentsOverview.xlsx)
|
|
|
+{8} Type of stimulation
|
|
|
+{9} Start of individual pulses (in ms)
|
|
|
+{10} StimulationStart (in samples)
|
|
|
+{11} StimulationEnd (in samples)
|
|
|
+{12} tart of individual pulses (in samples)
|
|
|
+
|
|
|
+
|
|
|
+--------------------------------------------------------
|
|
|
+--------------------------------------------------------
|
|
|
+--- LFPdata
|
|
|
+
|
|
|
+folder structure containing local field potential data segments in subfolders RampStimulation and Baseline with .mat files for each recording (subsubfolders named by animalID from ExperimentsOverview.xlsx)
|
|
|
+
|
|
|
+-- Baseline_LFP.mat
|
|
|
+contains LFP data from baseline period of spontaneous activity (without stimulation) for all recording sites (see ExperimentsOverview.xlsx for area)
|
|
|
+- LFP, int16, contains LFP data for baseline period in microvolts (-10 to 0 min from BaselinEnd). Data is filtered 1 to 100 Hz and downsampled to fs=200 Hz
|
|
|
+- fs, double, contains downsampled sampling frequency (200 Hz)
|
|
|
+
|
|
|
+-- AcuteStim1_LFP.mat
|
|
|
+contains LFP data from 30 repetitions of ramp light stimulations (AcuteStim1, 473 nm) for all recording sites (see ExperimentsOverview.xlsx for area and stimulation info)
|
|
|
+- LFP, int16, contains LFP data for stimulation periods in microvolts (-6 to 3 s from StimulationEnd). Data is filtered 1 to 100 Hz and downsampled to fs=200 Hz
|
|
|
+- fs, double, contains downsampled sampling frequency (200 Hz)
|
|
|
+
|
|
|
+- AcuteStim2_LFP.mat
|
|
|
+contains LFP data from 30 repetitions of control ramp light stimulations (AcuteStim2, 594 nm) for all recording sites (see ExperimentsOverview.xlsx for area and stimulation info)
|
|
|
+LFP contains LFP data for stimulation periods (-6 to 3 s from StimulationEnd). Data is filtered 1 to 100 Hz and downsampled to fs=200 Hz
|
|
|
+fs contains downsampled sampling frequency (200 Hz)
|
|
|
+
|
|
|
+
|
|
|
+--------------------------------------------------------
|
|
|
+--------------------------------------------------------
|
|
|
+--- sp.mat
|
|
|
+
|
|
|
+structure
|
|
|
+single unit data for 3554 single units
|
|
|
+
|
|
|
+- sp.st
|
|
|
+cell
|
|
|
+spike times per single unit in samples (sampling rate 32000 Hz)
|
|
|
+(times and samples refer to start of recording as sample 1)
|
|
|
+
|
|
|
+- sp.Nexp
|
|
|
+double
|
|
|
+experiment number per single unit (see column B in file ExperimentsOverview.xlsx)
|
|
|
+
|
|
|
+- sp.area
|
|
|
+double
|
|
|
+area per single unit
|
|
|
+1, for sp.peakchannel 17:20, ipsilateral mPFC hemisphere to stimulation, shank 1 (most medial)
|
|
|
+2, for sp.peakchannel 21:24, ipsilateral mPFC hemisphere to stimulation, shank 2
|
|
|
+3, for sp.peakchannel 25:28, ipsilateral mPFC hemisphere to stimulation, shank 3
|
|
|
+4, for sp.peakchannel 29:32, ipsilateral mPFC hemisphere to stimulation, shank 4 (most lateral)
|
|
|
+5, for sp.peakchannel 1:4, contralateral mPFC hemisphere to stimulation, shank 1 (most medial)
|
|
|
+6, for sp.peakchannel 5:8, contralateral mPFC hemisphere to stimulation, shank 2
|
|
|
+7, for sp.peakchannel 9:12, contralateral mPFC hemisphere to stimulation, shank 3
|
|
|
+8, for sp.peakchannel 13:16, contralateral mPFC hemisphere to stimulation, shank 4 (most lateral)
|
|
|
+
|
|
|
+- sp.peakchannel
|
|
|
+double
|
|
|
+peak channel (recording site) per single unit
|
|
|
+
|
|
|
+- sp.wf
|
|
|
+double
|
|
|
+average waveform per single unit (97 samples per single unit centered around negative peak, sampling rate 32000 Hz)
|
|
|
+
|
|
|
+- sp.age
|
|
|
+double
|
|
|
+age of recorded mouse per single unit
|
|
|
+
|
|
|
+-sp.FS
|
|
|
+logical
|
|
|
+0 if regular spiking 1 if fast spiking
|
|
|
+per single unit
|